Lanthanides and Quantum Dots as Forster Resonance Energy Transfer Agents for Diagnostics and Cellular Imaging
Lanthanides and Quantum Dots as Forster Resonance Energy Transfer Agents for Diagnostics and Cellular Imaging
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DOI：
10.1021/ic4017883
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发表时间：
2014-02-17
影响因子：
4.6
通讯作者：
Hildebrandt, Niko
中科院分区：
文献类型：
--
作者：
Geissler, Daniel;Linden, Stina;Hildebrandt, Niko
发光镧系元素标记物（LLL）和半导体量子点（QD）是两类非常特殊的（至少部分）无机荧光团，它们为Forster共振能量转移（FRET）提供了独特的性质。FRET是激发的供体荧光团和基态受体荧光团之间的能量转移过程，非常接近（约1-20 nm），因此非常适合于光学光谱和显微镜中的生物传感应用。在这一令人信服的审查，我们将概述的主要物理学的优势LLLs和量子点和它们的特殊性质的FRET。然后，我们将集中在一些最近的应用程序从FRET生物传感文献中使用LLLs作为供体和量子点作为供体和受体结合其他几个荧光团。最近的例子结合LLLs和量子点的光谱和时间复用从单步到多步FRET证明了这种独特的FRET对的通用和强大的生物传感能力。由于这篇综述发表在成像和传感论坛上，我们还将介绍我们小组的一些新结果，涉及基于LLL的时间门控细胞成像与光学三功能抗体和基于LLL-to-QD FRET的均相夹心免疫检测癌胚抗原。
Luminescent lanthanide labels (LLLs) and semiconductor quantum dots (QDs) are two very special classes of (at least partially) inorganic fluorophores, which provide unique properties for Forster resonance energy transfer (FRET). FRET is an energy-transfer process between an excited donor fluorophore and a ground-state acceptor fluorophore in close proximity (approximately 1-20 nm), and therefore it is extremely well suited for biosensing applications in optical spectroscopy and microscopy. Within this cogent review, we will outline the main photophysical advantages of LLLs and QDs and their special properties for FRET. We will then focus on some recent applications from the FRET biosensing literature using LLLs as donors and QDs as donors and acceptors in combination with several other fluorophores. Recent examples of combining LLLs and QDs for spectral and temporal multiplexing from single-step to multistep FRET demonstrate the versatile and powerful biosensing capabilities of this unique FRET pair. As this review is published in the Forum on Imaging and Sensing, we will also present some new results of our groups concerning LLL-based time-gated cellular imaging with optically trifunctional antibodies and LLL-to-QD FRET-based homogeneous sandwich immunoassays for the detection of carcinoembryonic antigen.