INHIBITION OF COLLAGENASE ACTIVITY BY N-CHLOROTAURINE, A PRODUCT OF ACTIVATED NEUTROPHILS
INHIBITION OF COLLAGENASE ACTIVITY BY N-CHLOROTAURINE, A PRODUCT OF ACTIVATED NEUTROPHILS
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DOI：
10.1002/art.1780370317
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发表时间：
1994-03-01
影响因子：
--
通讯作者：
DAVIES, KJA
中科院分区：
文献类型：
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作者：
DAVIES, JMS;HORWITZ, DA;DAVIES, KJA
Objective.研究N-氯牛磺酸对胶原酶活性的影响，作为中性粒细胞活化在炎症性关节炎中作用的模型。胶原酶对胶原的降解通过从H-3-胶原释放酸溶性计数来测量。N-氯牛磺酸抑制细菌胶原酶降解胶原。这一结果是由胶原酶的直接抑制/失活解释的，因为N-氯牛磺酸对胶原蛋白本身的蛋白水解敏感性没有影响。由于N-氯丙氨酸、N-氯亮氨酸和HOCl/OCl-均不能抑制胶原酶，因此这种作用似乎是N-氯牛磺酸所特有的;事实上，N-氯丙氨酸和N-氯亮氨酸实际上增加了胶原的蛋白水解敏感性。N-氯牛磺酸可以通过抑制/灭活胶原酶来最大限度地减少炎症性关节炎对软骨关节结构的损伤。
Objective. To study the effects of N-chlorotaurine on collagenase activity, as a model of the effects of neutrophil activation in inflammatory arthritis.Methods. Collagen degradation by collagenase was measured by the release of acid-soluble counts from H-3-collagen.Results. N-chlorotaurine inhibited the degradation of collagen by bacterial collagenase. This result is explained by a direct inhibition/inactivation of collagenase, since N-chlorotaurine had no effect on the proteolytic susceptibility of collagen itself. The effect appears to be specific to N-chlorotaurine since N-chloroaranine, N-chloroleucine, and HOCl/OCl- failed to inhibit collagenase; in fact, N-chloroalanine and N-chloroleucine actually increased the proteolytic susceptibility of collagen.Conclusion. N-chlorotaurine may minimize damage to cartilaginous joint structures in inflammatory arthritis by inhibiting/inactivating Collagenase.