In vitro cytotoxic, genotoxic, and oxidative effects of acyclic sesquiterpene farnesene
In vitro cytotoxic, genotoxic, and oxidative effects of acyclic sesquiterpene farnesene
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DOI：
10.3906/biy-1309-55
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发表时间：
2014-01-01
影响因子：
2.2
通讯作者：
Taspinar, Numan
中科院分区：
文献类型：
--
作者：
Celik, Kubra;Togar, Basak;Taspinar, Numan
法尼烯（FNS）是一种无环倍半萜。它具有广泛的重要生物效应，如抗氧化、抗菌和抗真菌特性，尽管其细胞毒性、细胞遗传学和氧化作用尚未在人体血液组织中进行研究。为此，采用MTT和乳酸脱氢酶（LDH）测定来评估细胞活力和细胞毒性。总抗氧化能力（TAC）和总氧化应激（TOS）参数评估氧化改变。此外，微核和染色体畸变试验用于诱变和基因毒性研究。结果显示，浓度大于100 μ g/mL时，FNS可降低细胞活力。所有检测的FNS浓度均无基因毒性。此外，与对照组相比，FNS体外处理导致培养血细胞中TAC水平升高，但未改变TOS水平。本研究结果表明，FNS可作为一种抗氧化化合物资源，在食品和药品工业中具有应用价值。
Farnesene (FNS) is an acyclic sesquiterpene. It has a wide range of important biological effects such as antioxidant, antimicrobial, and antifungal properties, although its cytotoxic, cytogenetic, and oxidative effects have not been investigated in human blood tissue yet. To this aim, both MTT and lactate dehydrogenase (LDH) assays were carried out to evaluate cell viability and cytotoxicity. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to assess oxidative alterations. In addition, micronucleus and chromosomal aberration tests were used for mutagenic and genotoxic studies. The results revealed that FNS reduced cell viability at concentrations of higher than 100 mu g/mL. All tested concentrations of FNS were found to be nongenotoxic. In addition, the in vitro treatments with FNS led to increases of TAC levels in cultured blood cells without changing TOS levels as compared to the control group. Our results demonstrate that FNS could be used as an antioxidant compound resource that may have applications in the food and drug industries.