A 2ND REGULATORY GENE, BLARL, ENCODING A POTENTIAL PENICILLIN-BINDING PROTEIN REQUIRED FOR INDUCTION OF BETA-LACTAMASE IN BACILLUS-LICHENIFORMIS
A 2ND REGULATORY GENE, BLARL, ENCODING A POTENTIAL PENICILLIN-BINDING PROTEIN REQUIRED FOR INDUCTION OF BETA-LACTAMASE IN BACILLUS-LICHENIFORMIS
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DOI：
10.1128/jb.169.9.3873-3878.1987
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发表时间：
1987-09-01
影响因子：
3.2
通讯作者：
LAMPEN, JO
中科院分区：
文献类型：
--
作者：
KOBAYASHI, T;ZHU, YF;LAMPEN, JO
第二个调控位点（blaR1）是诱导β所需的。对地衣芽孢杆菌749的-内酰胺酶合成进行了克隆和测序。该基因位于一个5.2千碱基对的SphI DNA片段上，该片段也含有。-内酰胺酶（blaP）和抑制因子（blaI）基因。枯草芽孢杆菌BD224携带这三个基因合成了。而携带该基因的大肠杆菌HB101没有显示出任何可检测到的酶诱导。通过亚克隆和DNA测序，鉴定出blaR1基因的开放阅读框为1803个碱基。该基因从blaI终止密码子下游2个碱基开始，前面是一个假定的间隔为5个碱基的Shine-Dalgarno序列（AAGGA）。推导出的blaR1产物（含601个氨基酸）分子量为68425。从疏水性剖面预测了5个跨膜区域。ph - ala - pro - ala - ser - thr - tyrr - lys（氨基酸398 - 405）周围的区域似乎位于膜外，与青霉素结合蛋白（包括β -内酰胺酶）的结合区域同源。400 ~ 421段的22个氨基酸与大肠杆菌PBP 2的青霉素结合区同源性超过70%。blaR1基因编码一种潜在的青霉素受体，这是诱导β所需的。地衣芽孢杆菌-内酰胺酶749。
A second regulatory locus (blaR1) required for the induction of .beta.-lactamase synthesis in Bacillus licheniformis 749 was cloned and sequenced. The gene was located on a 5.2-kilobase-pair SphI DNA fragment which also contained the .beta.-lactamase (blaP) and repressor (blaI) genes. Bacillus subtilis BD224 carrying these three genes synthesized .beta.-lactamase on exposure to cephalosporin C, whereas Escherichia coli HB101 carrying the genes did not show any detectable induction of the enzyme. An open reading frame of 1,803 bases was identified as the blaR1 gene by subcloning and DNA sequencing. The gene started 2 bases downstream of the termination codon of blaI and was preceded by a putative Shine-Dalgarno sequence (AAGGA) with a spacing of 5 bases. The deduced blaR1 product (601 amino acids) had a molecular weight of 68,425. Five transmembrane regions were predicted from the hydrophobicity profile. The region around Phe-Ala-Pro-Ala-Ser-Thr-Tyr-Lys (amino acids 398 to 405), which appeared to be located outside the membrane, was homologous to the binding regions of penicillin-binding proteins, including the .beta.-lactamases. The segment of 22 amino acids from 400 to 421 showed more than 70% homology to the penicillin-binding region of PBP 2 of E. coli. The blaR1 gene encodes a potential penicillin receptor which is required for the induction of .beta.-lactamase in B. licheniformis 749.