Selection of common markers for bone marrow stromal cells from various bones using real-time RT-PCR: Effects of passage number and donor age
Selection of common markers for bone marrow stromal cells from various bones using real-time RT-PCR: Effects of passage number and donor age
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DOI：
10.1089/ten.2006.0340
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发表时间：
2007-10-01
影响因子：
--
通讯作者：
Kato, Yukio
中科院分区：
文献类型：
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作者：
Igarashi, Akira;Segoshi, Kazumi;Kato, Yukio
骨髓基质细胞（BMSCs）在组织工程和细胞治疗中具有重要价值，但细胞的质量对治疗效果至关重要。为了测试可移植细胞的质量和身份，我们鉴定了在BMSC中比在成纤维细胞中表达水平更高的分子标志物。我们使用来自胫骨、股骨、髂骨和颌骨的众多BMSC系，以及皮肤和牙龈成纤维细胞，使用DNA微阵列和低密度阵列卡比较这些细胞的基因表达谱。胫骨和股骨BMSC的分化潜能与髂骨BMSC的分化潜能相似，而与颌骨BMSC不同，但所有BMSC系都具有许多共同的标记物，这些标记物在BMSC中的表达水平远高于成纤维细胞;几种BMSC标记物在颌骨和其他BMSC之间显示出离散的表达模式。这些共同的标记物在常规检测中可能有用，但它们的有效性可能取决于传代次数或供体年龄。在我们的研究中，传代数显着改变了几个标志物的表达水平，而供体年龄对它们的影响不大。考虑到BMSCs的体内位置和传代、表达水平增加的幅度和个体间差异的影响，我们在许多候选者中鉴定了几种可靠的标记物- LIF、IGF 1、PRG 1、MGP、BMP 4、CTGF、KCTD 12、IGFBP 7、TRIB 2和DYNC 1 I1。该标记物集可用于组织工程和细胞治疗中BMSCs的常规检测。
Bone marrow stromal cells (BMSCs) are valuable in tissue engineering and cell therapy, but the quality of the cells is critical for the efficacy of therapy. To test the quality and identity of transplantable cells, we identified the molecular markers that were expressed at higher levels in BMSCs than in fibroblasts. Using numerous BMSC lines from tibia, femur, ilium, and jaw, together with skin and gum fibroblasts, we compared the gene expression profiles of these cells using DNA microarrays and low-density array cards. The differentiation potential of tibia and femur BMSCs was similar to that of iliac BMSCs, and different from jaw BMSCs, but all BMSC lines had many common markers that were expressed at much higher levels in BMSCs than in fibroblasts; several BMSC markers showed discrete expression patterns between jaw and other BMSCs. The common markers are probably useful in routine tests, but their efficacy may depend upon the passage number or donor age. In our study the passage number markedly altered the expression levels of several markers, while donor age had little effect on them. Considering the effects of in vivo location of BMSCs and passage, magnitude of increase in expression levels, and interindividual differences, we identified several reliable markers - LIF, IGF1, PRG1, MGP, BMP4, CTGF, KCTD12, IGFBP7, TRIB2, and DYNC1I1 - among many candidates. This marker set may be useful in a routine test for BMSCs in tissue engineering and cell therapy.