Oxidative Stress in Pregnant Women Between 12 and 20 Weeks of Gestation and Preterm Birth.
Oxidative Stress in Pregnant Women Between 12 and 20 Weeks of Gestation and Preterm Birth.
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DOI：
10.1097/nnr.0000000000000414
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发表时间：
2020
期刊：
影响因子：
2.5
通讯作者：
Zimmerman MC
中科院分区：
文献类型：
--
作者：
Moore TA;Samson K;Ahmad IM;Case AJ;Zimmerman MC
氧化应激与早产（PTB）之间存在已知的关系。然而，很少有研究对妊娠早期或中期的氧化应激进行前瞻性测量，也没有研究使用电子顺磁共振（EPR）光谱前瞻性预测PTB。本研究的目的是确定抗氧化剂和活性氧（ROS）之间的预测关系，特别是，超氧化物（），过氧亚硝酸盐（ooo−），羟基自由基（●OH），使用EPR光谱，在妊娠12至20周测量，并与PTB的发病率进行比较。从三级围产中心招募的孕妇（n = 140）获得血液。用EPR光谱直接分析全血ROS、ooo−和●OH。红细胞裂解液用于测定抗氧化剂。PTB定义为妊娠<37周分娩。ROS、OONO−或●OH与PTB发病率之间没有差异。过氧化氢酶活性、谷胱甘肽和还原性/氧化性谷胱甘肽比值显著降低。逻辑回归表明，孕妇过氧化氢酶活性降低与早产几率增加有关。我们前瞻性地比较了抗氧化剂和特异性ROS在妊娠12 - 20周的孕妇中与PTB发病率的关系。结果很少，但确实表明抗氧化剂-特别是降低过氧化氢酶活性-在妊娠早期可能与PTB有关；然而，这些发现应谨慎解释，可能没有临床意义。
A known relationship exists between oxidative stress and preterm birth (PTB). However, few studies have measured oxidative stress prospectively in early or midpregnancy, and no studies have used electron paramagnetic resonance (EPR) spectroscopy prospectively to predict PTB. The purpose of this study was to identify predictive relationships between antioxidants and reactive oxygen species (ROS), specifically, superoxide (), peroxynitrite (OONO−), and hydroxyl radical (●OH), using EPR spectroscopy, measured between 12 and 20 weeks of gestation and compare with the incidence of PTB. Blood was obtained from pregnant women (n = 140) recruited from a tertiary perinatal center. Whole blood was analyzed directly for ROS, , OONO−, and ●OH using EPR spectroscopy. Red blood cell lysate was used to measure antioxidants. PTB was defined as parturition at <37 weeks of gestation. No differences were found between ROS, , OONO−, or ●OH with the incidence of PTB. Catalase activity, glutathione, and reduced/oxidized glutathione ratio were significantly lower with PTB. Logistic regression suggests decreased catalase activity in pregnant women is associated with increased odds of delivering prematurely. We prospectively compared antioxidants and specific ROS using EPR spectroscopy in pregnant women between 12 and 20 weeks of gestation with the incidence of PTB. Results are minimal but do suggest that antioxidants—specifically decreased catalase activity—in early pregnancy may be associated with PTB; however, these findings should be cautiously interpreted and may not have clinical significance.