Molecular mapping of a major gene conferring resistance to maize mosaic virus
Molecular mapping of a major gene conferring resistance to maize mosaic virus
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DOI：
10.1007/s001220050559
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发表时间：
1997-07-01
影响因子：
5.4
通讯作者：
McMullen, MD
中科院分区：
文献类型：
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作者：
Ming, R;Brewbaker, JL;McMullen, MD
本研究的目的是确定玉米花叶病毒（MMV）抗性的遗传基础。利用分子标记技术对91个玉米（Zea mays L.）重组近交系（RILs），来源于Hi31（对MMV具有抗性的B68转化）和Ki14（对MMV敏感的泰国近交系）之间的杂交。1993年冬季和1994年夏季在夏威夷的病圃中对RIL进行了MMV抗性评价。选择28个高度敏感的RILs进行基因定位，采用混合抽样方法。来自合并DNA的初步证据表明，3号染色体上靠近着丝粒的限制性片段长度多态性（RFLP）探针偏向易感亲本等位基因。在103个RFLP位点的91个RILs的分析证实存在一个主要的MMV抗性基因的染色体3。该基因座上的抗性等位基因，以前称为Mv1，存在于抗性亲本Hi31中，并可追溯到用于赋予B68普通锈病抗性的阿根廷亲本。我们的结论是，在B68和加勒比提示MMV抗性涉及一个主效基因MV1位于3号染色体上的RFLP标记umc102和php20508之间。
The objective of this study was to determine the genetic basis of resistance to maize mosaic virus (MMV). Molecular markers were used to map resistance loci to MMV in a set of 91 maize (Zea mays L.) recombinant inbred lines (RILs), derived from the cross between Hi31 (a B68 conversion resistant to MMV) and Ki14 (a Thai inbred susceptible to MMV). The RILs were evaluated for MMV resistance in disease nurseries in Hawaii in the winter of 1993 and the summer of 1994. Twenty-eight highly susceptible RILs were chosen for gene mapping using the pooled-sampling approach. Initial evidence from the pooled DNA indicated that restriction fragment length polymorphism (RFLP) probes on chromosome 3 near the centromere were biased to the susceptible parent allele. Analysis of 91 RILs at 103 RFLP loci confirmed the presence of a major MMV resistance gene on chromosome 3. The resistant allele at this locus, previously named Mv1, is present in the resistant parent Hi31 and traces back to the Argentine parent used in conferring common rust resistance to B68. We conclude that resistance to MMV in B68 and Caribbean hints involves a major gene mv1 on chromosome 3 located between RFLP markers umc102 and php20508.