Anti-Androgenic Activity of Icarisid II from Epimedium Herb in Prostate Cancer LNCaP Cells
Anti-Androgenic Activity of Icarisid II from Epimedium Herb in Prostate Cancer LNCaP Cells
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DOI：
10.3177/jnsv.61.201
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发表时间：
2015-04-01
影响因子：
1.6
通讯作者：
Mikamo, Hiroshige
中科院分区：
文献类型：
--
作者：
Miura, Yoko;Oyama, Masayoshi;Mikamo, Hiroshige
抗雄激素被认为是治疗前列腺癌的潜在治疗剂。我们确定，淫羊藿（EH）提取物显示出抗雄激素活性的荧光素酶测定使用雄激素受体阳性前列腺癌LNCaP细胞。九个EH衍生的黄酮类化合物进行了检查。结果确定淫羊藿苷II是一种非常有效的抗雄激素EH衍生的黄酮类化合物。定量RT-PCR分析证实，黄酮醇抑制雄激素反应性KLK 3基因的表达。
Anti-androgens are regarded as potential therapeutic agents for the treatment of prostate cancer. We determined that an epimedium herb (EH) extract exhibited anti-androgenic activity in a luciferase assay using androgen receptor-positive prostate cancer LNCaP cells. Nine EH-derived flavonoids were examined. The results identified icarisid II as a very potent anti-androgenic EH-derived flavonoid. A quantitative RT-PCR analysis confirmed that the flavonol suppressed the expression of the androgen-responsive KLK3 gene.