Mechanism of taurine reducing inflammation and organ injury in sepsis mice
Mechanism of taurine reducing inflammation and organ injury in sepsis mice
复制标题
牛磺酸减轻脓毒症小鼠炎症和器官损伤的机制
DOI：
10.1016/j.cellimm.2022.104503
复制
发表时间：
2022-04-11
影响因子：
4.3
通讯作者：
Zhu, Liuluan
中科院分区：
文献类型：
--
作者：
Ma, Yuan;Zhang, Yue;Zhu, Liuluan
脓毒症发病机制的根本基础是炎症失衡，这被认为是治疗的主要目标。牛磺酸是一种细胞内游离氨基酸，具有抗炎和抗氧化作用。为探讨牛磺酸对脓毒症的保护作用机制，本研究采用体内外实验研究牛磺酸对中性粒细胞和单核细胞免疫功能的影响。代谢组学分析表明，大量的牛磺酸在中性粒细胞和单核细胞和牛磺酸水平显着降低LPS暴露后。补充牛磺酸可降低LPS刺激的中性粒细胞和单核细胞中肿瘤坏死因子-α（TNF-α）、白细胞介素-1 β（IL-1 β）的表达，并通过限制活性氧减少中性粒细胞胞外陷阱的形成。此外，牛磺酸保护脓毒症小鼠免于死亡，通过减少中性粒细胞浸润和TNF-α产生改善肺、肝和肾中的组织损伤。我们的数据表明，补充牛磺酸可能是一个有前途的治疗策略，脓毒症，以减少过度炎症和改善多器官功能障碍。
The fundamental basis for the pathogenesis of sepsis is an inflammatory imbalance, which is considered to be the main target for treatment. Taurine is an intracellular free amino acid that has anti-inflammatory and antioxidant effects. To investigate the protective mechanism of taurine in sepsis, we used in vitro and in vivo experiments to explore the effects of taurine on neutrophil and monocyte immune function. Metabolomic analysis showed large amounts of taurine in neutrophils and monocytes and a dramatic decrease in taurine levels after LPS exposure. Taurine supplementation decreased the expressions of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) in LPS-challenged neutrophils and monocytes and reduced the formation of neutrophil extracellular traps by restricting reactive oxygen species. Moreover, taurine protected septic mice from death, improved tissue injuries in the lung, liver, and kidney by reducing neutrophil infiltration and TNF-alpha production. Our data indicate that a supplement with taurine might be a promising therapeutic strategy for sepsis to reduce hyper inflammation and improve multi-organ dysfunctions.