Lipozyme TL IM-catalyzed Esterification of Oleic Acid for 1,3-Diacylglycerol Preparation
Lipozyme TL IM-catalyzed Esterification of Oleic Acid for 1,3-Diacylglycerol Preparation
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DOI：
10.7503/cjcu20141106
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发表时间：
2015-08-10
影响因子：
1
通讯作者：
Liu Dehua
中科院分区：
文献类型：
--
作者：
Wang Zitian;Su Jianxiao;Liu Dehua
探讨了脂肪酶tlim介导油酸与单甘油酯酯化制备1,3-二油基甘油（DO）的可能性，并系统考察了溶剂、水活度、温度、油酸与单硬脂（MO）的摩尔比和溶剂用量对脂肪酶的DO产率和1,3位置选择性的影响。在最佳条件下(45℃，1。5 g Lipozyme TL IM，油酸与MO的摩尔比为1。5 1 6。0克氯仿，水活度为0。在1,3-DO含量大于92%的条件下，总DO收率可达80%。Lipozyme TL IM在体系中表现出较好的操作稳定性，连续使用5批后，脂肪酶的活性和选择性均未出现明显下降。
The potential of using lipase Lipozyme TL IM-mediated esterification of oleic acid with monoglyceride for 1,3-dioleoylglycerol(DO) preparation was explored and the effects of solvent, water activity, temperature, molar ratio of oleic acid to monostearin(MO) and the solvent quantity on the DO yield and 1,3-positional selectivity of the lipase were investigated systematically. Under the optimum conditions (45 degrees C, 1. 5 g of Lipozyme TL IM, molar ratio of oleic acid to MO is 1. 5 1, 6. 0 g chloroform, and water activity is 0. 33), a total DO yield of 80% could be achieved with the content of 1,3-DO over 92%. Lipozyme TL IM showed rather good operational stability in the system and there was no obvious reduction in lipase's activity and selectivity even after being continually used for 5 batches.