Silver nanoparticle and lysozyme/tannic acid layer-by-layer assembly antimicrobial multilayer on magnetic nanoparticle by an eco-friendly route
Silver nanoparticle and lysozyme/tannic acid layer-by-layer assembly antimicrobial multilayer on magnetic nanoparticle by an eco-friendly route
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DOI：
10.1016/j.msec.2017.03.192
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发表时间：
2017-07-01
影响因子：
7.9
通讯作者：
Xing, Xiaodong
中科院分区：
文献类型：
--
作者：
Wang, Xi;Cao, Weiwei;Xing, Xiaodong
采用一种简单、经济、绿色的合成方法制备了具有抗菌功能的银纳米磁性纳米复合材料。在该合成中，两种天然化合物，带正电荷的溶菌酶（Lys）和带负电荷的单宁酸（TA），交替沉积在Fe 3 O 4纳米粒子（IONP）表面的层-层（LbL）自组装技术。然后通过原位还原Ag+的方法将AgNPs包埋起来，实现对革兰氏阳性菌和革兰氏阴性菌的互补抗菌功能。其中，AgNPs的沉积可以在没有任何外部还原剂的情况下容易地实现。系统的抗菌试验表明，合成的纳米复合材料对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌都具有较高的抗菌效率。抗菌机理的研究表明，这些纳米复合材料可以导致细菌细胞膜的解体和细胞质内容物的泄漏。此外，细胞质膜的通透性改变可能有助于纳米复合材料释放的Ag+进入细胞，并进一步导致细菌死亡。由于IONP具有优异的磁响应性能，因此在消毒后的应用环境中，该纳米复合材料可以通过外磁场容易地恢复。通过利用这些性能，开发的纳米复合材料可能是一个理想的候选人与有前途的抗菌应用。(C)2017爱思唯尔B. V.保留所有权利。
A facile, economical and green synthetic route was developed to fabricate magnetic nanocomposite arming with silver nanoparticles (AgNPs) for antibacterial application. In this synthesis, two natural compounds, positively charged lysozyme (Lys) and negatively charged tannic acid (TA), were alternately deposited on Fe3O4 nanoparticles (IONPs) surface by layer-by-layer (LbL) self-assembly technique. And then AgNPs were embedded by an in situ reduction of Ag+ so as to achieve complementary antibacterial functions to act against Gram-positive and Gram-negative bacteria. In which, the deposition of AgNPs can be facilely achieved without any external reducing agent. The systematic antibacterial assays showed that synthesized nanocomposites had high antibacterial efficiency against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. Investigation of antimicrobial mechanism suggested that these nanocomposites could lead to the disorganization of bacterial cytomembrane and leakage of cytoplasmic contents. Moreover, the permeable alteration of cytoplasmic membrane may facilitate the Ag+ released from nanocomposite entering into cells, and further cause the bacterial death. Due to the excellent magnetic responsive performance of IONPs, the nanocomposites can be easy recovery by external magnetic field from application environment after disinfection. By taking advantages of such properties, the developed nanocomposite could be an ideal candidate with promising antibacterial applications. (C) 2017 Elsevier B.V. All rights reserved.