Protein motion from non-specific to specific DNA by three-dimensional routes aided by supercoiling
Protein motion from non-specific to specific DNA by three-dimensional routes aided by supercoiling
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DOI：
10.1093/emboj/cdg125
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发表时间：
2003-03-17
期刊：
影响因子：
11.4
通讯作者：
Halford, SE
中科院分区：
文献类型：
--
作者：
Gowers, DM;Halford, SE
通常认为DNA结合蛋白通过首先随机与DNA结合，然后通过沿DNA的一维（1D）扩散沿着易位到特定位点来定位其靶位点。我们在这里报告说，非特异性DNA运送蛋白质到它们的靶位点，以及当保持在目标附近的连锁时，当与目标共线。因此，与流行的观点相反，蛋白质主要通过三维（3D）而不是一维途径，通过单个DNA分子内的多个解离/重新缔合事件从随机位点移动到特定位点。我们还发现了DNA超螺旋在靶位点定位中的作用。蛋白质在超螺旋DNA中比在松弛DNA中更容易找到它们的位点，这再次表明3D而不是1D途径。
DNA-binding proteins are generally thought to locate their target sites by first associating with the DNA at random and then translocating to the specific site by one-dimensional (1D) diffusion along the DNA. We report here that non-specific DNA conveys proteins to their target sites just as well when held near the target by catenation as when co-linear with the target. Hence, contrary to the prevalent view, proteins move from random to specific sites primarily by three-dimensional (3D) rather than 1D pathways, by multiple dissociation/re-association events within a single DNA molecule. We also uncover a role for DNA supercoiling in target-site location. Proteins find their sites more readily in supercoiled than in relaxed DNA, again indicating 3D rather than 1D routes.