Expression of three Rx homeobox genes in embryonic and adult zebrafish
Expression of three Rx homeobox genes in embryonic and adult zebrafish
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DOI：
10.1016/s0925-4773(99)00077-5
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发表时间：
1999-06-01
影响因子：
2.6
通讯作者：
Raymond, PA
中科院分区：
文献类型：
--
作者：
Chuang, JC;Mathers, PH;Raymond, PA
Rx基因在眼的发育过程中起重要作用。在这项研究中，我们分析了三个斑马鱼Rx基因（Zrx 1，2，3）在胚胎和成人的表达模式。这三个基因都表现出动态的时空表达模式。Zrx 3最早在神经板的最前区域中表达，在产生腹侧间脑和视网膜的区域中。随着发育的进行，Zrx 3的表达在外侧视原基中减少，并且在视杯中不存在，但是保留在间脑的腹侧中线，并且在成人的下丘脑中表达。随着神经视网膜开始分化，Zrx 3在内核层的细胞亚群中重新表达，推测为双极细胞，并且这种表达在成人中保留。相反，Zrx 1/2的表达开始稍晚，最初与Zrx 3一致，但随后变为互补。保留在视原基上，但从间脑腹侧中线消失。随着视网膜的分化，Zrx 1/2下调，除了在外核层中，它们继续在视锥细胞中高水平表达，而不是视杆细胞。光感受器这是第一个转录因子的描述，区分锥和杆光感受器。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
Thr paired-class homeobox gene, Rx, is important in eye development. In this study we analyze expression patterns of three zebrafish Rx genes (Zrx1, 2, 3) in embryos and adults. All three genes show dynamic spatiotemporal patterns of expression. Zrx3 is expressed earliest, in the anteriormost region of the neural plate, in regions that give rise to ventral diencephalon and retinae. As development proceeds, Zrx3 expression is reduced in the lateral optic primordia, and is absent in the optic cup, but is retained at the ventral midline of the diencephalon, and is expressed in hypothalamus in the adult. As the neural retina begins to differentiate, Zrx3 is re-expressed in a subset of cells in the inner nuclear layer, presumably bipolar cells, and this expression is retained in the adult. In contrast, Zrx1/2 have a slightly later onset of expression, are initially coincident with Zrx3, but then become complementary. remaining on in the optic primordia but disappearing from the ventral midline of the diencephalon. Zrx1/2 are down-regulated as the retina differentiates, except in the outer nuclear layer where they continue to be expressed at high levels in cone, bur not rod. photoreceptors. This is the first transcription factor described that distinguishes between cone and rod photoreceptors. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.