A Wireless Electro-Optic Headstage with Digital Signal Processing and Data Compression for Multimodal Electrophysiology and Optogenetic Stimulation
A Wireless Electro-Optic Headstage with Digital Signal Processing and Data Compression for Multimodal Electrophysiology and Optogenetic Stimulation
复制标题
具有数字信号处理和数据压缩功能的无线光电探头，用于多模态电生理学和光遗传学刺激
DOI：
--
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
B. Gosselin
中科院分区：
文献类型：
--
作者：
G. Bilodeau;Gabriel Gagnon;L. L. Gagnon;C. Ethier;I. Timofeev;B. Gosselin
本文提出了一种无线电光探头，用于在执行多通道光刺激的同时并行记录神经动作电位（AP）、局部场电位（LFP）和肌电图（EMG）。探头设计用于对自由移动的啮齿动物（如小型实验室小鼠）进行实验。它可以记录多达 32 个通道上的电生理活动，并仅使用商用现成 (COTS) 组件来刺激多达 4 个光学通道。嵌入式数字现场可编程门阵列 (FPGA) 用于实现 AP 和 LFP 波形的实时数字分离以及 EMG 记录。它还通过 AP 检测和压缩执行数据缩减，允许从 32 个并行通道无线收集和传输数据。数字滤波器经过优化可减少延迟，因此 32 个通道可在单个数据路径上进行时间复用，从而降低功耗并优化资源利用率。执行信号分离可以压缩 AP 并抽取 LFP/EMG。这将传输的数据量减少了 7.77 倍（发射速率为 50 AP/秒），并允许两个信号有更高的通道数。主机重量仅为 3.8 克（含 100 mAh 电池），重量为 4.68 克（含塑料包装和开关）。
This paper presents a wireless electro-optic headstage for parallel electrophysiological recording of neural action potential (AP), local field potentials (LFP) and electromyography (EMG), while performing multichannel optical stimulation. The headstage is designed for enabling experiments with freely moving rodents, like small laboratory mice. It can record electrophysiological activity on up to 32 channels and stimulate with up to 4 optical channels using only commercial off the shelf (COTS) components. An embedded digital field-programmable gate array (FPGA) is used to allow real time digital separation of AP and LFP waveform and the recording of EMG. It also performs data reduction through AP detection and compression allowing to collect and transmit data wirelessly from 32 parallel channels. The digital filters are optimized to reduce latency so the 32 channels can be time multiplexed on a single data path to decrease power consumption and optimize resource utilisation. Performing signal separation allows the compression of the AP and the decimation of the LFP/EMG. This reduces the amount of data to transmit by a factor of 7.77 (for a firing rate of 50 AP/second) and allow a higher channel count for both signals. The headstage only weights 3.8 g w/ a 100 mAh battery and 4.68 g with the plastic packaging and on/off switch.