AMPEROMETRIC MONITORING OF STIMULATED CATECHOLAMINE RELEASE FROM RAT PHEOCHROMOCYTOMA (PC12) CELLS AT THE ZEPTOMOLE LEVEL
AMPEROMETRIC MONITORING OF STIMULATED CATECHOLAMINE RELEASE FROM RAT PHEOCHROMOCYTOMA (PC12) CELLS AT THE ZEPTOMOLE LEVEL
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DOI：
10.1021/ac00091a007
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发表时间：
1994-10-01
影响因子：
7.4
通讯作者：
EWING, AG
中科院分区：
文献类型：
--
作者：
CHEN, TK;LUO, GO;EWING, AG
用碳纤维微电极直流安培检测法，在zeptomole水平上观察到大鼠嗜铬细胞瘤（PC 12）细胞释放的儿茶酚胺。已记录并分析了来自PC 12细胞的时间分辨的单个胞吐事件，时间分辨率为1.2 ms。1912电流瞬变下的平均面积从13 PC 12细胞对应于190 zmol（114 - 300分子每个释放事件）。这些电流瞬变的半高处的平均宽度为9.3 ms，与胞吐的时间框架一致。本文报道的方法的检测限低至31 zmol。这是首次直接电化学观察PC 12细胞量子释放的报道。这种电化学方案的成功应用，以监测从小泡释放的儿茶酚胺也表明，它可能是可能的应用这种技术来监测从突触囊泡的量子释放。
Catecholamine release from rat pheochromocytoma (PC12) cells has been observed at zeptomole levels using dc-amperometric detection with carbon fiber microelectrodes. Time-resolved individual exocytic events from PC12 cells have been recorded and analyzed with 1.2 ms time resolution. The average area under 1912 current transients from 13 PC12 cells corresponds to 190 zmol (114 300 molecules per release event). The average width at half-height of these current transients is 9.3 ms, in agreement with the time frame of exocytosis. The detection limit of the method reported here is as low as 31 zmol. This is the first report of direct electrochemical observation of quantal release from PC12 cells. The successful application of this electrochemical scheme to monitor catecholamines released from small vesicles also suggests that it may be possible to apply this technique to monitor quantal release from synaptic vesicles.