Epstein-Barr virus early antigen diffuse (EBV-EA/D)-directed immunoglobulin A antibodies in systemic lupus erythematosus patients
Epstein-Barr virus early antigen diffuse (EBV-EA/D)-directed immunoglobulin A antibodies in systemic lupus erythematosus patients
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DOI：
10.3109/03009742.2012.665944
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发表时间：
2012-01-01
影响因子：
2.1
通讯作者：
Duus, K.
中科院分区：
文献类型：
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作者：
Draborg, A. H.;Jorgensen, J. M.;Duus, K.
目的：探讨系统性红斑狼疮(SLE)患者对EB病毒(EBV)溶血循环抗原的血清学反应是否发生改变。方法：采用酶联免疫吸附试验(EL ISA)检测60例SLE患者、40例硬皮病(SSC)患者、20例原发性干燥综合征(PSS)患者、20例类风湿关节炎(RA)患者、20例正常对照和20例正常对照血清中EBV早期抗原弥漫性(EBV-EA/D)抗体。结果：SLE患者免疫球蛋白(Ig)A、Ig G和Ig M EBV-EA/D抗体滴度显著高于健康对照组，这一结果不能用免疫抑制治疗或疾病活动性来解释。以IgA、EBV-EA/D抗体差异最大(P=0.0013)，SLE患者血清阳性率为58%，健康对照组为0。RA和SSc患者及抗SCL-70血清阳性者的IgA EBV-EA/D抗体滴度均升高(分别为40%，P=0.014；60%，P=0.015；38.5%，P=0.045)。然而，滴度一般低于SLE患者。结论：我们的研究结果支持EBV与SLE之间的关联。在SLE患者中发现EBV-EA/D导向的IgA抗体滴度升高，提示EBV在上皮细胞中重新激活或在B细胞重新激活后再感染，提示潜伏感染缺乏控制。
Objective: We sought to determine whether the serological response towards lytic cycle antigens of Epstein Barr virus (EBV) is altered in systemic lupus erythematosus (SLE) patients.Method: We used enzyme-linked immunosorbent assay (ELISA) to investigate the prevalence of EBV early antigen diffuse (EBV-EA/D) antibodies in sera from 60 patients with SLE, 40 with scleroderma (SSc), 20 with primary Sjogren's syndrome (pSS), 20 with rheumatoid arthritis (RA), 20 healthy controls, and also subjects with various circulating autoantibodies. Samples from patients were obtained from clinics specialized within the diseases in Denmark and Sweden and samples from healthy controls were obtained from volunteers.Results: A significant elevated titre of immunoglobulin (Ig)A, IgG, and IgM EBV-EA/D antibodies was found in SLE patients compared to healthy controls, a finding not explained by immunosuppressive treatment or disease activity. The largest difference was observed for IgA EBV-EA/D antibodies (p = 0.0013) with a seropositive rate of 58% in SLE patients and 0% in healthy controls. RA and SSc patients and individuals seropositive for anti-Scl-70 were additionally found to have elevated titres of IgA EBV-EA/D antibodies (40%, p = 0.014; 60%, p = 0.015; and 38.5%, p = 0.045, respectively). However, the titres were generally lower than in SLE patients.Conclusion: Our findings support an association between EBV and SLE. The elevated titre of EBV-EA/D-directed IgA antibodies found in SLE patients could suggest reactivation of EBV in epithelial cells or reinfection of epithelial cells after reactivation in B cells, indicating lack of control of the latent infection.