Antigenic and genotypical characterization of Newcastle disease viruses isolated in Taiwan between 1969 and 1996
Antigenic and genotypical characterization of Newcastle disease viruses isolated in Taiwan between 1969 and 1996
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DOI：
10.1016/j.vetmic.2004.09.005
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发表时间：
2004-11-30
影响因子：
3.3
通讯作者：
Alexander, DJ
中科院分区：
文献类型：
--
作者：
Tsai, HJ;Chang, KH;Alexander, DJ
在过去的三十年里，台湾发生了三次纽卡斯尔病（ND）大流行（1969年、1984年和1995年）。为了更好地了解台湾过去ND流行病之间的关系，对1969年至1996年分离的36株ND病毒（NDV）进行了抗原性和基因型特征分析。这些病毒的抗原性通过其引起小鼠单克隆抗体（mAb）与分离株感染的细胞培养物结合的能力进行分析。使用一组22个单克隆抗体将NDV分成亚组，总共揭示了18种结合模式。还测定了覆盖这些分离物的融合蛋白基因的切割位点的序列。系统发育分析结果将36个NDV分为1、11、VIb、VIIa、VIII和两个新基因型（暂命名为X和VIh）。1969年的强毒分离株为基因型X和VIh; 1984-1985年的强毒分离株为基因型VIb、VIh、VIIa和X;而1995-1996年的强毒分离株为基因型VIIa或VIII。1969年和1984年的部分强毒株具有相同的单克隆抗体结合模式和基因型，1995-1996年的强毒株的单克隆抗体结合模式未见报道。结论是不同基因型和抗原型的NDV在台湾至少自1969年以来已经共同传播。此外，1969年和1984年分离的菌株之间存在动物流行病学联系，而1995-1996年的流行是由新的抗原变体引起的。(C)2004 Elsevier B. V.保留所有权利。
Three major epidemics of Newcastle disease (ND) occurred in Taiwan over the past three decades (in 1969, 1984, and 1995). In order to gain a better understanding of the relationships between past ND epizootics in Taiwan, 36 ND viruses (NDVs) isolated between 1969 and 1996 were characterized antigenically and genotypically. The antigenicity of these viruses was analysed by their ability to cause binding of mouse monoclonal antibodies (mAbs) to cell cultures infected with the isolate. Using a panel of 22 mAbs to divide NDVs into subgroups, a total of 18 binding patterns were revealed. The sequences covering the cleavage site of the fusion protein gene of these isolates were also determined. The results of the phylogenetic analysis placed 36 NDVs into 1, 11, VIb, VIIa, VIII and two novel genotypes (provisionally termed X and VIh). The 1969 velogenic isolates were of genotypes X and VIh; the 1984-1985 velogenic isolates were genotyped VIb, VIh, VIIa, and X; while the 1995-1996 velogenic isolates were genotyped VIIa or VIII. Some 1969 and 1984 velogenic isolates were of the same mAbs binding pattern and genotype, and the mAbs binding patterns of the 1995-1996 isolates have not been seen before. It is concluded that velogenic NDVs of different genotype and antigenic type have co-circulated in Taiwan at least since 1969. Also there were epizootiological links between strains isolated in 1969 and 1984, whereas the 1995-1996 epidemic was caused by new antigenic variants. (C) 2004 Elsevier B.V. All rights reserved.