DEVELOPMENTAL POTENTIAL AND DYNAMIC BEHAVIOR OF HEMATOPOIETIC STEM-CELLS
DEVELOPMENTAL POTENTIAL AND DYNAMIC BEHAVIOR OF HEMATOPOIETIC STEM-CELLS
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DOI：
10.1016/0092-8674(86)90566-0
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发表时间：
1986-06-20
期刊：
影响因子：
64.5
通讯作者：
MULLIGAN, RC
中科院分区：
文献类型：
--
作者：
LEMISCHKA, IR;RAULET, DH;MULLIGAN, RC
我们已经使用逆转录病毒介导的基因转移标记造血干细胞在体外，并跟踪这些细胞移植到致命的辐射受体后的命运。证明了几类干细胞，包括其后代完全重新填充所有造血谱系的细胞以及主要贡献于某些谱系或特定解剖位置的细胞。在大多数受体中，我们发现很少（1或2个）干细胞克隆占成熟造血细胞的大多数。这些结果加上再移植的研究表明，在体内机制的时间控制干细胞的使用。进一步的研究表明，正常的造血是由不同的干细胞克隆的顺序激活而不是整个干细胞库的平均贡献。
We have used retrovirus-mediated gene transfer to mark hematopoietic stem cells in vitro and have tracked the fate of these cells after their transplantation into lethally irradiated recipients. Several classes of stem cells are demonstrated, including cells whose progeny completely repopulate all hematopoietic lineages as well as cells that contribute predominantly to certain lineages or to specific anatomical locations. In a majority of recipients, we find that few (1 or 2) stem-cell clones account for the majority of the mature hematopoietic cells. These results coupled with retransplantation studies suggest an in vivo mechanism for the temporal control of stem-cell use. Further studies based on periodic sampling of primary recipients suggest that normal hematopoiesis results from the sequential activation of different stem-cell clones rather than from an averaged contribution of the entire stem-cell pool.