Haloferax volcanii Flagella Are Required for Motility but Are Not Involved in PibD-Dependent Surface Adhesion
Haloferax volcanii Flagella Are Required for Motility but Are Not Involved in PibD-Dependent Surface Adhesion
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Haloferax volcanii 鞭毛是运动所必需的，但不参与 PibD 依赖性表面粘附
DOI：
10.1128/jb.00133-10
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发表时间：
2010
影响因子：
3.2
通讯作者：
M. Pohlschröder
中科院分区：
文献类型：
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作者：
M. Tripepi;Saheed Imam;M. Pohlschröder
虽然火山嗜盐菌的基因组中含有编码鞭毛蛋白的基因（flgA 1-flgA 2）和其他编码鞭毛组装蛋白的基因，但以前的报道认为，火山嗜盐菌的基因组中含有鞭毛蛋白的基因。火山是不动的。与这些报告相反，我们现在已经确定了H。火山是能动的。此外，我们还确定了一个H。缺乏鞭毛蛋白基因的volcanii缺失突变体是不运动的。然而，与包括其他古细菌在内的其他原核生物的鞭毛特征不同，H.火山鞭毛似乎在表面粘附中不起重要作用。虽然鞭毛通常在细菌和古细菌中发挥类似的功能作用，但古细菌鞭毛生物合成中涉及的过程与组装细菌鞭毛中涉及的过程不同，而是与产生细菌IV型皮利中涉及的过程相似。与这一观察结果一致，我们已经确定，除了破坏前鞭毛蛋白加工，删除pibD，编码前鞭毛蛋白肽酶，阻止其他H。volcanii IV型菌毛蛋白样蛋白。此外，除了消除游泳运动性之外，并且与flgA 1-flgA 2缺失不同，缺失pibD消除了H. volcanii粘附在玻璃表面，表明非鞭毛IV型菌毛样结构在H. volcano表面附着力。
ABSTRACT Although the genome of Haloferax volcanii contains genes (flgA1-flgA2) that encode flagellins and others that encode proteins involved in flagellar assembly, previous reports have concluded that H. volcanii is nonmotile. Contrary to these reports, we have now identified conditions under which H. volcanii is motile. Moreover, we have determined that an H. volcanii deletion mutant lacking flagellin genes is not motile. However, unlike flagella characterized in other prokaryotes, including other archaea, the H. volcanii flagella do not appear to play a significant role in surface adhesion. While flagella often play similar functional roles in bacteria and archaea, the processes involved in the biosynthesis of archaeal flagella do not resemble those involved in assembling bacterial flagella but, instead, are similar to those involved in producing bacterial type IV pili. Consistent with this observation, we have determined that, in addition to disrupting preflagellin processing, deleting pibD, which encodes the preflagellin peptidase, prevents the maturation of other H. volcanii type IV pilin-like proteins. Moreover, in addition to abolishing swimming motility, and unlike the flgA1-flgA2 deletion, deleting pibD eliminates the ability of H. volcanii to adhere to a glass surface, indicating that a nonflagellar type IV pilus-like structure plays a critical role in H. volcanii surface adhesion.
影响因子：
--
作者：
G. O’Toole;L. Pratt;P. Watnick;D. Newman;Valerie B. Weaver;R. Kolter
通讯作者：
G. O’Toole;L. Pratt;P. Watnick;D. Newman;Valerie B. Weaver;R. Kolter
影响因子：
11.4
作者：
BLYN, LB;BRAATEN, BA;LOW, DA
通讯作者：
LOW, DA