G-reactive protein decreases expression of thrombomodulin and endothelial protein C receptor in human endothelial cells
G-reactive protein decreases expression of thrombomodulin and endothelial protein C receptor in human endothelial cells
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DOI：
10.1016/j.surg.2005.06.003
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发表时间：
2005-08-01
期刊：
影响因子：
3.8
通讯作者：
Chen, CY
中科院分区：
文献类型：
--
作者：
Nan, BC;Yang, H;Chen, CY
背景资料。C反应蛋白(CRP)与动脉粥样硬化和血栓形成有关。然而，目前尚不清楚CRP是否对内皮细胞的抗血栓形成特性有直接影响。本研究旨在探讨C反应蛋白(CRP)对人血管内皮细胞血栓调节蛋白(TM)和内皮蛋白C受体(EPCR)表达的影响。人冠状动脉内皮细胞(HCAECs)经C反应蛋白(CRP)处理后呈剂量和时间依赖性。用实时荧光定量聚合酶链式反应检测TM和EPCR的信使RNA水平。用抗CD32抗体和姜黄素阻断CRP的潜在作用。在HCAEC中，CPLP(10和25mU g/m L)使TM信使RNA水平分别比对照组降低18%和30%(P
Background. C-reactive protein (CRP) is associated with atherosclerosis and thrombosis. However, it is unclear whether CRP has direct effects on the antithrombogenic Properties of endothelial cells. The objective of the present study was to determine the effect of CRP on the expression of thrombomodulin (TM) and the endothelial protein C receptor (EPCR) in human endothelial cells.Methods. Human coronary artery endothelial cells (HCAECs) were treated with CRP in a dosed and time-dependent manner. The messenger RNA levels of TM and EPCR were determined by real-time polymerase chain reaction. Anti-CD32 antibody and curcumin were used to block the potential effects of CRPResults. In HCAECs, CPLP (10 and 25 mu g/mL) significantly reduced TM messenger RNA levels by 18 and 30%, respectively, compared with controls (P