A reduction in the butyrate producing species Roseburia spp. and Faecalibacterium prausnitzii is associated with chronic kidney disease progression
A reduction in the butyrate producing species Roseburia spp. and Faecalibacterium prausnitzii is associated with chronic kidney disease progression
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DOI：
10.1007/s10482-016-0737-y
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发表时间：
2016-10-01
影响因子：
2.6
通讯作者：
Chen, Ye
中科院分区：
文献类型：
--
作者：
Jiang, Shuanghong;Xie, Shan;Chen, Ye
人类肠道微生物群在人类健康中起着重要作用，也可能与肾脏疾病有关。最近，人们对丁酸盐产生菌的兴趣有所增加，这是一种对慢性肾脏疾病（CKD）中的粪便状况知之甚少。因此，我们评估了丁酸生产物种罗斯拜瑞氏菌属的差异。和普氏粪杆菌。对65例CKD患者和20例健康对照进行病例对照研究。使用定量实时聚合酶链反应（qPCR）对差异进行定量验证。采用斯皮尔曼等级相关分析肠道菌群与临床变量之间的相关性。罗斯拜瑞氏菌属和F. prausnitzii在CKD患者和对照组中存在显著差异（分别为p = 0.001; p = 0.025），并且在终末期肾病（分别为p = 0.000; p = 0.003）和微炎症（分别为p = 0.004; p = 0.001）中降低更明显。罗斯拜瑞氏菌属和F.与血浆C反应蛋白（r =-0.493，p = 0.00; r =-0.528，p = 0.000）和胱抑素C（r =-0.321，p = 0.006; r =-0.445，p = 0.000）呈负相关。它们与eGFR正相关（分别为r = 0.347，p = 0.002; r = 0.416，p = 0.000）。罗斯拜瑞氏菌属、F. prausnitzii和CRP以及肾功能表明，丁酸产生细菌的消耗可能有助于CKD相关炎症和CKD进展。罗斯拜瑞氏菌属和F.因此，prausnitzii可以用作“微生物标记物”。
The human gut microbiota plays an important role in human health and might also be implicated in kidney disease. The interest in butyrate producing bacteria has recently increased and is a poorly understood faecal condition in chronic kidney disease (CKD). Therefore, we evaluated differences of the butyrate producing species Roseburia spp. and Faecalibacterium prausnitzii in the faeces of Chinese patients with CKD. A case-control study was carried out for 65 CKD patients and 20 healthy controls. Differences were quantitatively validated using quantitative real-time polymerase chain reaction (qPCR). Spearman rank correlation was used to analyse the correlation between gut microbiota and clinical variables. Roseburia spp. and F. prausnitzii were significantly different in CKD patients and controls (p = 0.001; p = 0.025, respectively) and reduced more markedly in end stage renal disease (p = 0.000; p = 0.003, respectively) and microinflammation (p = 0.004; p = 0.001, respectively). Roseburia spp. and F. prausnitzii were negatively associated with C-reactive protein in plasma (r = -0.493, p = 0.00; r = -0.528, p = 0.000; respectively) and Cystatin C (r = -0.321, p = 0.006; r = -0.445, p = 0.000; respectively). They were positively associated with eGFR (r = 0.347, p = 0.002; r = 0.416, p = 0.000; respectively). The negative correlation between Roseburia spp., F. prausnitzii and CRP and renal function suggested that the depletion of butyrate producing bacteria may contribute to CKD-associated inflammation and CKD progression. Roseburia spp. and F. prausnitzii may thus serve as 'microbiomarkers'.