Cell Growth Suppression of Astrocytoma C6 Cells by Glial Fibrillary Acidic Protein cDNA Transfection
Cell Growth Suppression of Astrocytoma C6 Cells by Glial Fibrillary Acidic Protein cDNA Transfection
复制标题
胶质纤维酸性蛋白 cDNA 转染对星形细胞瘤 C6 细胞生长的抑制
DOI：
10.1046/j.1471-4159.1994.63051975.x
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发表时间：
1994
影响因子：
4.7
通讯作者：
K. Uyemura
中科院分区：
文献类型：
--
作者：
M. Toda;Masayuki Miura;H. Asou;S. Toya;K. Uyemura
摘要：包括胶质细胞酸性蛋白（GFAP）在内的中间丝家族的细胞功能除了作为细胞的结构元件外，还不太清楚。GFAP的表达在星形胶质细胞中是特异性的，并且受到发育调节，表明其参与星形胶质细胞的细胞生长和分化。我们将鼠GFAP cDNA转染到大鼠星形细胞瘤C6细胞系中，以评估GFAP对细胞的特异性作用。两种稳定的GFAP转染的细胞系GFC 6 - 5和GFC 6 - 6表现出一系列形态和生长特征，这些特征将它们与其对应物区分开来，即，仅用新霉素抗性基因转染的NeoC 6细胞和天然C6细胞。GFC 6 - 5和GFC 6 - 6细胞均表现出细长的细胞形状，具有富含GFAP的延伸突起，显著抑制细胞生长，并降低溴脱氧尿苷摄取。Western blot分析显示，与NeoC 6和C6相比，GFAP在GFC 6 - 5和GFC 6 - 6中的表达显著增加，与所有细胞系中相似的波形蛋白表达相反。结果表明，GFAP的表达对C6细胞的细胞形态和细胞生长抑制有显著影响，提示GFAP可能在星形细胞瘤中起抑癌作用。
Abstract: The cellular functions of the intermediate filament family including glial fibrillary acidic protein (GFAP) are not well known yet beyond their roles as structural elements of cells. Expression of GFAP, which is specific in astrocytes and regulated developmentally, suggests its involvement in cell growth and differentiation of astrocytes. We transfected murine GFAP cDNA into a rat astrocytoma C6 cell line to assess the specific effect of GFAP on cells. Two stable GFAP‐transfected cell lines, GFC6‐5 and GFC6‐6, exhibited a series of morphological and growth characteristics that distinguish them from their counterparts, i.e., NeoC6 cells transfected only with the neomycin‐resistant gene, and native C6 cells. Both GFC6‐5 and GFC6‐6 cells showed elongated cell shapes with extended processes rich in GFAP, markedly suppressed cell growth, and decreased bromodeoxyuridine uptake. Western blot analysis revealed a remarkable increase of GFAP expression in GFC6‐5 and GFC6‐6 compared with that in NeoC6 and C6, in contrast to similar vimentin expression in all cell lines. The results indicate that the expression of GFAP has dramatic effects on cell morphology and cell growth suppression in C6 cells, suggesting that GFAP may function as a tumor suppressor in astrocytoma.