Comparison of peroxidase response to mental arithmetic stress in saliva of smokers and non-smokers
Comparison of peroxidase response to mental arithmetic stress in saliva of smokers and non-smokers
复制标题
DOI：
10.2131/jts.32.121
复制
发表时间：
2007-05-01
影响因子：
2
通讯作者：
Takagi, Kuniaki
中科院分区：
文献类型：
--
作者：
Goi, Nobuhiro;Hirai, Yuuko;Takagi, Kuniaki
唾液是第一种遇到外源性物质或气体（如香烟烟雾（CS））的体液。本研究的目的是检查是否吸烟影响口腔过氧化物酶（OPO）的反应精神压力。受试者为39名非吸烟者和10名吸烟者。在实验中，作为心理应激源的Kraepelin心理诊断测试和唾液在测试开始前30分钟、测试开始前、测试开始后即刻和测试开始后30分钟取样。除了尿酸浓度、流速、伊加、硫氰酸盐（SCN-）浓度、作为唾液应激标志物的淀粉酶活性和指示唾液抗氧化和抗菌能力的超弱化学发光（UCL）水平之外，还测量吸烟者和非吸烟者之间对测试的OPO反应性。此外，我们还研究了吸烟对心理应激反应中唾液过氧化物酶（SPO）和髓过氧化物酶（MPO）活性的影响。结果表明，不吸烟组伊加、淀粉酶、SCN-、UCL水平均高于吸烟组，且不吸烟组在Kraepelin试验后伊加和UCL水平明显升高。吸烟者的SCN-水平高于非吸烟者，并且在所有阶段中，非吸烟组的OPO活性都更大。此外，只有非吸烟者在测试后不久MPO活性显著增加。MPO可能在急性心理应激反应中起重要作用，而CS可显著抑制这种反应。
Saliva is the first body fluid to encounter exogenous materials or gases such as cigarette smoke (CS). The aim of this study was to examine whether smoking affects oral peroxidase (OPO) reactivity to mental stress. The subjects were 39 non-smokers and 10 smokers. In the experiment, the Kraepelin psychodiagnostic test as a psychological stressor and saliva was sampled 30 min before, just before, immediately after, and 30 min after the beginning of the test. OPO reactivity to the test between smokers and non-smokers was measured in addition to uric acid concentration, flow rate, IgA, thiocyanate (SCN-) concentration, amylase activity as a salivary stress marker, and ultra-weak chemiluminescence (UCL) level, which is indicative of salivary antioxidative and antibacterial abilities. Moreover, we Studied the effect of smoking on the response of salivary peroxidase (SPO) and myeloperoxidase (MPO) activity to mental stress, respectively. The results Showed that the IgA concentration, amylase activity, SCN- concentration, and UCL level are higher in the non-smoking group than smoking group and the IgA concentration and UCL level increased in the non-smokers significantly just after the Kraepelin test. The levels of SCN- were higher in smokers than in non-smokers and OPO activity was greater in the non-smoking group in all sessions. Furthermore, only the non-smokers had significantly increased MPO activity just after the test. MPO may play a crucial role in the response to acute psychological stress besides inflammation, and CS suppresses this response significantly.