OLIGOCLONAL LYMPHOCYTES-T IN THE CEREBROSPINAL-FLUID OF PATIENTS WITH MULTIPLE-SCLEROSIS
OLIGOCLONAL LYMPHOCYTES-T IN THE CEREBROSPINAL-FLUID OF PATIENTS WITH MULTIPLE-SCLEROSIS
复制标题
DOI：
10.1084/jem.167.4.1313
复制
发表时间：
1988-04-01
影响因子：
15.3
通讯作者：
WEINER, HL
中科院分区：
文献类型：
--
作者：
HAFLER, DA;DUBY, AD;WEINER, HL
我们研究了慢性进展型多发性硬化（MS）患者脑脊液（CSF）中的T细胞群。在扩增前克隆来自CSF和血液的单个T细胞，并通过分析重排的T细胞受体β来确定它们的克隆型。链和γ连锁基因对2例慢性进展性MS患者的87个血液和CSF T细胞克隆进行了常见TCR基因重排模式的检测。在一名患者中，28个CSF来源的T细胞克隆中有18个表现出共同的TCR基因重排，表明寡克隆T细胞群;在血液中，在26个T细胞克隆中发现两种模式。在另一名患者中，27个CSF来源的克隆中有5个具有共同的TCR基因重排模式。相比之下，没有共同的β。在来自血液或CSF或患有亚急性硬化性全脑炎的患者的67个T细胞克隆中，在来自患有带状疱疹脑膜脑炎的患者的CSF的20个克隆中，或在来自正常受试者的66个克隆中发现重排模式。还研究了1例持续时间为8个月的非典型致死性MS受试者，其CSF或血液中没有寡克隆T细胞。这些结果表明，不同的寡克隆T细胞群体可以在CSF免疫区室的受试者与非恶性炎症性疾病，他们可以创建一个新的途径，为调查的特异性T细胞反应的中枢神经系统内。
We have investigated the T cell populations in the cerebrospinal fluid (CSF) of chronic progressive multiple sclerosis (MS) patients. Individual T cells from the CSF nad blood were cloned before expansion and their clonotypes were defined by analysis of rearranged T cell receptor .beta. chain and .gamma. chain genes. 87 T cell clones from blood and CSF of two patients with chronic progressive MS were examined for common TCR gene rearrangement patterns. In one patient, 18 of 28 CSF-derived T cell clones demonstrated common TCR gene rearrangements indicating oligoclonal T cell populations; in the blood, two patterns were found twice among 26 T cell clones. In another patient, 5 of 27 CSF-derived clones had common TCR gene rearrangement patterns. In contrast, no common .beta. rearrangement pattern was found among 67 T cell clones derived from the blood or CSF or a patient with subacute scleroising panencephalitis, among 20 clones from the CSF of a patient with herpes zoster meningoencephalitis, or among 66 clones from a normal subject. A subject with atypical, fatal MS of 8-mo duration was also studied and did not have oligoclonal T cells in the CSF or blood. These results demonstrate that distinct oligoclonal T cell populations can be found in the CSF immune compartment of subjects with nonmalignant inflammatory disease and they can create a new avenue for the investigation of the specificity of the T cell response within the central nervous system.