A specific Streptococcus mutans strain aggravates non-alcoholic fatty liver disease
A specific Streptococcus mutans strain aggravates non-alcoholic fatty liver disease
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DOI：
10.1111/odi.12191
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发表时间：
2014-10-01
期刊：
影响因子：
3.8
通讯作者：
Nakano, K.
中科院分区：
文献类型：
--
作者：
Naka, S.;Nomura, R.;Nakano, K.
目的变形链球菌是一种主要的龋齿病原体，与脑出血和炎症性肠病的加重有关。在这项研究中，我们在小鼠模型中评估了变形链球菌对非酒精性脂肪性肝炎（NASH）发展的影响。材料与方法采用变形链球菌口服菌株MT8148 （c血清型）和血液分离株TW871 (k)。C57BL/6J小鼠（6周龄）饲喂高脂饲料4周；然后静脉注射试验菌株或磷酸盐缓冲盐水。小鼠在8周或12周后被安乐死。测定全身、切除肝脏和内脏脂肪重量，并对肝脏标本进行组织病理学评估。结果TW871感染小鼠的体重和肝脏质量明显高于MT8148或磷酸盐缓冲盐水感染小鼠。组织病理学分析显示，感染TW871 8周后，肝脏中炎症细胞明显浸润，脂肪细胞沉积；12周后切除的肝脏也观察到纤维化。结论一种特定的突变s.t ans菌株可诱发NASH。
ObjectiveStreptococcus mutans, a major dental caries pathogen, has shown to be associated with the aggravation of cerebral hemorrhage and inflammatory bowel diseases. In this study, we evaluated the effects of S.mutans on the development of non-alcoholic steatohepatitis (NASH) in a mouse model.Materials and methodsStreptococcus mutans oral strain MT8148 (serotype c) and a blood isolate TW871 (k) were used. C57BL/6J mice (6weeks old) were fed a high-fat diet for 4weeks; the test strains or phosphate-buffered saline was then intravenously administered. Mice were euthanized after 8 or 12weeks. Whole body, extirpated liver, and visceral fat weights were determined, and histopathological evaluations of the liver specimens were performed.ResultsMice infected with TW871 showed significantly greater body and liver weights than those administered MT8148 or phosphate-buffered saline. Histopathological analyses revealed prominent infiltration of inflammatory cells and adipocellular deposition in livers extirpated 8weeks after an infection with TW871; fibrosis was also observed in livers extirpated after 12weeks.ConclusionThese results suggest that a specific strain of S.mutans could induce NASH.