TARGETED MUTATIONS IN THE CAENORHABDITIS-ELEGANS POU HOMEO BOX GENE CEH-18 CAUSE DEFECTS IN OOCYTE CELL-CYCLE ARREST, GONAD MIGRATION, AND EPIDERMAL DIFFERENTIATION
TARGETED MUTATIONS IN THE CAENORHABDITIS-ELEGANS POU HOMEO BOX GENE CEH-18 CAUSE DEFECTS IN OOCYTE CELL-CYCLE ARREST, GONAD MIGRATION, AND EPIDERMAL DIFFERENTIATION
复制标题
DOI：
10.1101/gad.8.16.1935
复制
发表时间：
1994-08-15
影响因子：
10.5
通讯作者：
RUVKUN, G
中科院分区：
文献类型：
--
作者：
GREENSTEIN, D;HIRD, S;RUVKUN, G
我们使用靶向基因失活来分析秀丽隐杆线虫POU基因ceh-18的功能，并在体内解剖其功能结构域。在ceh-18突变体中，卵母细胞在减数分裂前期I的终变期表现出不完全的穿透失败，而是经历多轮DNA复制而没有胞质分裂。CEH-18在向卵母细胞发出信号的性腺鞘细胞中表达，但在卵母细胞中不表达。这表明ceh-18直接或间接影响鞘细胞信号，导致卵母细胞维持终变期停滞。CEH-18还参与引导性腺迁移和在胚后发育期间指定表皮细胞的分化表型。通过单独缺失POUhd或POUsp选择性地破坏一半POU结构域的靶向缺失的分析表明，每个CEH-18 POU亚结构域足以在体内具有部分活性。
We used targeted gene inactivation to analyze the function of a Caenorhabditis elegans POU gene, ceh-18, and to dissect its functional domains in vivo. In ceh-18 mutants, oocytes exhibit an incompletely penetrant failure to arrest in diakinesis of meiotic prophase I and instead undergo multiple rounds of DNA replication without cytokinesis. ceh-18 is expressed in the gonadal sheath cells that signal the oocyte, but not in the oocyte. This suggests that ceh-18 affects, directly or indirectly, a sheath cell signal that causes oocytes to maintain diakinesis arrest. ceh-18 also participates in directing gonad migration and in specifying the differentiated phenotypes of epidermal cells during postembryonic development. Analysis of targeted deletions that disrupt half of the POU domain selectively by deleting either the POUhd or the POUsp alone, indicates that each CEH-18 POU subdomain is sufficient for partial activity in vivo.