Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization
Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization
复制标题
仿生胶原-配体相互作用对原纤维内矿化的贡献
DOI：
10.1126/sciadv.aav9075
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发表时间：
2019-03-01
期刊：
影响因子：
13.6
通讯作者：
Niu, L. N.
中科院分区：
文献类型：
--
作者：
Song, Q.;Jiao, K.;Niu, L. N.
当代的纤维内矿化机制模型是用胶原原纤维作为模板建立的，没有考虑胶原结合的磷灰石成核抑制剂的贡献。然而，脊椎动物体内矿化的胶原基质含有用于纤维内矿化的结合基质蛋白。带负电荷的高分子量聚羧酸与重建的胶原交联，以创建一个模型，用于检查胶原-配体相互作用对纤维内矿化的贡献。低温电子显微镜和分子动力学模拟表明，在与胶原交联后，结合的聚电解质将预成核簇单线包裹成沿纤维表面的链状聚集体，增加了用于纤维内矿化的矿化前体池。与未改性支架在聚电解质稳定矿化溶液中的矿化相比，矿化支架的质量更高，生物力学性能更好。胶原-配体相互作用为非矿化组织异位钙化的发生和潜在原因提供了见解。
Contemporary models of intrafibrillar mineralization mechanisms are established using collagen fibrils as templates without considering the contribution from collagen-bound apatite nucleation inhibitors. However, collagen matrices destined for mineralization in vertebrates contain bound matrix proteins for intrafibrillar mineralization. Negatively charged, high-molecular weight polycarboxylic acid is cross-linked to reconstituted collagen to create a model for examining the contribution of collagen-ligand interaction to intrafibrillar mineralization. Cryogenic electron microscopy and molecular dynamics simulation show that, after cross-linking to collagen, the bound polyelectrolyte caches prenucleation cluster singlets into chain-like aggregates along the fibrillar surface to increase the pool of mineralization precursors available for intrafibrillar mineralization. Higher-quality mineralized scaffolds with better biomechanical properties are achieved compared with mineralization of unmodified scaffolds in polyelectrolyte-stabilized mineralization solution. Collagen-ligand interaction provides insights on the genesis of heterogeneously mineralized tissues and the potential causes of ectopic calcification in nonmineralized body tissues.