Paradoxical aging in HIV: immune senescence of B Cells is most prominent in young age
Paradoxical aging in HIV: immune senescence of B Cells is most prominent in young age
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DOI：
10.18632/aging.101229
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发表时间：
2017-04-01
期刊：
影响因子：
5.2
通讯作者：
Pahwa, Savita
中科院分区：
文献类型：
--
作者：
Rinaldi, Stefano;Pallikkuth, Suresh;Pahwa, Savita
联合抗逆转录病毒疗法（cART）可以使艾滋病毒感染者的预期寿命正常，年龄>50岁的人是增长最快的艾滋病毒群体。虽然艾滋病毒和衰老与体液免疫受损独立相关，但艾滋病毒感染者的免疫状态相对未被探索。在本研究中，使用流感疫苗接种来探测HIV阴性“健康对照”（HC）和病毒学控制的接受cART（HIV）的HIV感染参与者（n=124）的B细胞区室中年龄相关的扰动，按年龄分组为年轻（= 60岁）。在HC和HIV中，接种后第21天的H1N1抗体应答与年龄呈负相关。冷冻保存的PBMC的免疫表型表明，与年轻HC相比，老年HC中双阴性B细胞的频率增加，浆母细胞减少。值得注意的是，年轻的HIV与年轻的HC不同，但在B细胞表型、流感特异性自发（d7）或记忆（d21）抗体分泌细胞方面与老年HC相似。我们的结论是，B细胞免疫衰老是一个突出的现象，在年轻的艾滋病毒相比，年轻的HC，但老年艾滋病毒和老年HC之间的区别不太明显，虽然这两个群体表现出年龄相关的B细胞功能障碍。
Combination antiretroviral therapies (cART) can lead to normal life expectancy in HIV-infected persons, and people aged >50 yrs represent the fastest growing HIV group. Although HIV and aging are independently associated with impaired humoral immunity, immune status in people aging with HIV is relatively unexplored. In this study influenza vaccination was used to probe age associated perturbations in the B cell compartment of HIV-negative "healthy controls" (HC) and virologically controlled HIV-infected participants on cART (HIV) (n=124), grouped by age as young (= 60 yrs). H1N1 antibody response at d21 post-vaccination correlated inversely with age in both HC and HIV. Immunophenotyping of cryopreserved PBMC demonstrated increased frequencies of double negative B cells and decreased plasmablasts in old compared to young HC. Remarkably, young HIV were different from young HC but similar to old HC in B cell phenotype, influenza specific spontaneous (d7) or memory (d21) antibody secreting cells. We conclude that B cell immune senescence is a prominent phenomenon in young HIV in comparison to young HC, but distinctions between old HIV and old HC are less evident though both groups manifest age-associated B cell dysfunction.