Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China
Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China
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DOI：
10.1016/j.hal.2019.03.011
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发表时间：
2019-04-01
期刊：
影响因子：
6.6
通讯作者：
Yu, Ren-Cheng
中科院分区：
文献类型：
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作者：
Chen, Zhen-Fan;Zhang, Qing-Chun;Yu, Ren-Cheng
沿着2009年以来，渤海秦皇岛沿岸出现了由皮级浮游植物形成的大规模水华，严重抑制了养殖扇贝的摄食活动和生长。基于浮游植物样品的色素配置文件和克隆库分析在水华期间，主要水华形成物种主要被确定为浮游植物Aureococcus anophagefferens Hargraves et Sieburth，在美国和南非的密集的棕色潮汐的成因种。由于水华形成微藻的形态特征不明显，以及对浮游植物群落组成的了解有限，导致水华的种类仍存在争议。本研究利用18 S rDNA V4区高通量测序的方法，对2013年和2014年的浮游植物群落组成进行了研究。从秦皇岛近岸沃茨褐潮带浮游植物样品中鉴定出18个纲级真核微藻类群和2000多个操作分类单位（OTU）。这两年，A。在盛花期，以食心虫为优势种，其最大相对丰度超过60%。沿着其它证据进一步证实了A.浮游植物是渤海浮游植物水华的主要成因。2013 - 2014年秦皇岛近岸沃茨褐潮爆发具有较强的年际变化，甲藻优势度呈上升趋势。
Large-scale blooms formed by pico-sized phytoplankton, which strongly inhibited feeding activity and growth of cultured scallops, have been recorded along the coast of Qinhuangdao in the Bohai Sea since 2009. Based on pigment profiles and clone library analysis of phytoplankton samples during the blooms, the major bloom-forming species was primarily identified as pelagophyte Aureococcus anophagefferens Hargraves et Sieburth, the causative species of intensive brown tides in the United States and South Africa. Due to the indistinct morphological features of the bloom-forming microalgae and limited knowledge on the composition of phytoplankton communities, there were still disputes concerning the causative species of the blooms. In this study, the method of high-throughput sequencing targeted 18S rDNA V4 region was used to study the composition of pico- and nano-sized phytoplankton communities in 2013 and 2014. A total of 18 groups of eukaryotic microalgae at the class level and more than 2000 operational taxonomic units (OTUs) were identified in phytoplankton samples collected from the brown-tide zone in the Qinhuangdao coastal waters. For both years, A. anophagefferens was the most dominant species during the bloom period and its maximum relative abundance exceeded 60 percent. Along with other evidence, the results further confirm that A. anophagefferens is the major causative species of the pico-sized phytoplankton blooms in the Bohai Sea. The outbreak of brown tide exhibited a strong inter-annual variation between 2013 and 2014, and an increasing dominance of dinoflagellates could be observed in the Qinhuangdao coastal waters.