Fluorescent microsphere technique to measure cerebral blood flow in the rat
Fluorescent microsphere technique to measure cerebral blood flow in the rat
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DOI：
10.1038/nprot.2006.332
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发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Flameng, W.
中科院分区：
文献类型：
--
作者：
De Visscher, G.;Haseldonckx, M.;Flameng, W.
本方案描述了一种使用荧光微球和相同材料的结构评估平行测量脑血流量（CBF）的方法。该方法基于标准微球技术，栓塞毛细血管与血流量成正比，但需要溶解组织以回收微球。为了将血流与组织形态联系起来，我们修改了荧光微球技术，在冷冻或振动切片中量化，允许通过免疫组织化学或标准组织学进行结构分析。该协议需要8小时50分钟，没有停顿，完成，但额外的流量测量或特定的协议可能会增加所需的时间。
The present protocol describes a method for parallel measurement of cerebral blood flow (CBF) using fluorescent microspheres and structural assessment of the same material. The method is based on the standard microsphere technique, embolizing capillaries proportional to the blood flow, but requires dissolution of the tissue to retrieve the microspheres. To link the blood flow to the tissue morphology we modified the technique to fluorescent microspheres, which are quantified in cryo- or vibratome sections, allowing structural analysis by, for example, immunohistochemistry or standard histology. The protocol takes 8 h 50 min, without pauses, to complete, but additional flow measurements or specific protocols can increase the time needed.