Regularized phase tomography enables study of mineralized and unmineralized tissue in porous bone scaffold
Regularized phase tomography enables study of mineralized and unmineralized tissue in porous bone scaffold
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正则化相位断层扫描能够研究多孔骨支架中的矿化和非矿化组织
DOI：
10.1111/j.1365-2818.2009.03345.x
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发表时间：
2010
影响因子：
2
通讯作者：
Françoise Peyrin
中科院分区：
文献类型：
--
作者：
M. Langer;Y. Liu;F. Tortelli;Peter Cloetens;R. Cancedda;Françoise Peyrin
使用正则化相位断层扫描对体外细胞培养的多孔骨支架样品中的非钙化纤维基质进行成像。骨骼和骨支架的 3D 微结构先前已通过微型计算机断层扫描、同步辐射 (SR) 微型计算机断层扫描和微衍射进行了研究。然而，这些技术都无法解决低钙化的未成熟骨前纤维结构。 Skelete 多孔支架圆盘接种了成骨细胞（成骨细胞和前破骨细胞的组合），以及作为对照，仅接种了前破骨细胞，然后培养 8 周。随后使用基于 SR 传播的相差成像对它们进行成像。将使用正则化全息相位断层扫描方法的重建与标准（吸收）SR 微计算机断层扫描进行比较，结果表明可以对重建体积中的钙化部分和未成熟骨基质进行定量分析，例如体积和厚度测量。发现了这种类型的培养物对 Skelite 的影响的迹象，例如矿化的变化和成熟骨在支架壁上的沉积。结果通过组织学研究得到验证。
Regularized phase tomography was used to image non‐calcified fibrous matrix in in vitro cell‐cultivated porous bone scaffold samples. 3D micro‐architecture of bone and bone scaffold has previously been studied by micro‐computed tomography, synchrotron radiation (SR) micro‐computed tomography and microdiffraction. However, neither of these techniques can resolve the low‐calcified immature pre‐bone fibrous structures. Skelite porous scaffold discs were seeded with osteoblasts, a combination of osteoblast and pre‐osteoclasts and, as controls, with pre‐osteoclasts only, and then cultivated for 8 weeks. They were subsequently imaged using SR propagation‐based phase contrast imaging. Reconstructions using a regularized holographic phase tomography approach were compared to standard (absorption) SR micro‐computed tomography, which show that quantitative analysis, such as volume and thickness measurements, of both the calcified fraction and the immature bone matrix in the reconstructed volumes is enabled. Indications of the effect of this type of culture on Skelite, such as change in mineralization and deposit of mature bone on the walls of the scaffold, are found. The results are verified with a histological study.