Identification of a Primary Stroma and Novel Endothelial Cell Projections in the Developing Human Cornea
Identification of a Primary Stroma and Novel Endothelial Cell Projections in the Developing Human Cornea
复制标题
DOI：
10.1167/iovs.61.6.5
复制
发表时间：
2020-06-01
影响因子：
4.4
通讯作者：
Meek, Keith M.
中科院分区：
文献类型：
--
作者：
Feneck, Eleanor M.;Lewis, Philip N.;Meek, Keith M.
目的.研究人类角膜发育的初始事件，重点关注细胞迁移、细胞外基质合成和组织。目的探讨人角膜早期发育过程中细胞外基质中是否存在弹性纤维。从发育的第7周至第17周收集人角膜。弹性纤维增强染色，单宁酸-醋酸双氧铀，适用于所有组织。三维系列块面扫描电子显微镜结合传统的透射电子显微镜被用来分析角膜基质。从角膜发育的第7周起，在中央角膜中鉴定出具有正交排列的原纤维的脱细胞胶原原代基质。在第7.5周，间充质细胞向角膜中央迁移，并与脱细胞胶原基质结合。发现了来自内皮的新型细胞延伸。从角膜发育的第12周开始，发现弹性纤维集中在角膜后周边基质中。这项研究提供了新的证据，脱细胞的初级基质在胚胎人角膜的早期发育。细胞延伸作为通讯系统的一部分存在，并被假设为有助于间充质细胞的迁移和成熟角膜的发育。在早期角膜发育中发现的弹性纤维可能在建立角膜形状中起重要作用。
PURPOSE. To investigate the initial events in the development of the human cornea, focusing on cell migration, and extracellular matrix synthesis and organization. To determine whether elastic fibers are present in the extracellular matrix during early human corneal development.METHODS. Human corneas were collected from week 7 to week 17 of development. An elastic fiber-enhancing stain, tannic acid-uranyl acetate, was applied to all tissue. Three-dimensional serial block-face scanning electron microscopy combined with conventional transmission electron microscopy was used to analyze the corneal stroma.RESULTS. An acellular collagenous primary stroma with an orthogonal arrangement of fibrils was identified in the central cornea from week 7 of corneal development. At week 7.5, mesenchymal cells migrated toward the central cornea and associated with the acellular collagenous matrix. Novel cell extensions from the endothelium were identified. Elastic fibers were found concentrated in the posterior peripheral corneal stroma from week 12 of corneal development.CONCLUSIONS. This study provides novel evidence of an acellular primary stroma in the early development of the embryonic human cornea. Cell extensions exist as part of a communication system and are hypothesized to assist in the migration of the mesenchymal cells and the development of the mature cornea. Elastic fibers identified in early corneal development may play an important role in establishing corneal shape.