Genetic Analyses of Genus Cypripedium Found in Northern Japanese Islands and Related Species Endemic to Northeast China
Genetic Analyses of Genus Cypripedium Found in Northern Japanese Islands and Related Species Endemic to Northeast China
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日本北部列岛杓兰属及中国东北特有种的遗传分析
DOI：
10.2503/jjshs.74.234
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发表时间：
2005
影响因子：
--
通讯作者：
K. Yagi
中科院分区：
文献类型：
--
作者：
Shin;M. Ochiai;K. Furuta;K. Yagi
对日本列岛和中国东北地区的几个种和变种的Cypripedium进行了遗传分析。C.麦兰索斯湾hotei-atsmorianum和C.麦兰索斯湾rebunense是在日本北方采集的。它们被认为是C.产于欧亚大陆的大花木兰。虽然叶绿体核酮糖-1，5-二磷酸脱羧酶/氧化酶大亚基（rbcL）在种间没有差异，但C.麦兰索斯湾hotei-atsmorianum和其他两个被发现在核DNA编码的核糖体RNA的内转录间隔区（ITS）的序列。随机扩增多态性DNA（RAPD）分析表明，C.麦兰索斯湾从别人的报复。梭calceolus和C. rbcL分析和ITS序列分析表明，欧亚大陆的大花木兰属植物之间存在差异。遗传分析证实C.× ventricosum是C.盖盖霉C. macranthos。因此，利用rbcL和ITS区的分析可以有效地区分Cypripedium属植物的种和变种。
Several species and varieties of genus Cypripedium grown in Japanese Islands and northeast China were investigated using genetic analysis. C. macranthos var. hotei-atsmorianum and C. macranthos var. rebunense were collected in northern Japan. They are regarded as varieties or forms of C. macranthos of Eurasia. Although the large subunit of ribulose-1, 5-diphosphate decarboxylase/oxydase (rbcL) of chloroplast did not show any difference among the species, a difference between C. macranthos var. hotei-atsmorianum and the other two was found in the sequence of nuclear DNA encoding the internal transcribed spacer (ITS) region of ribosomal RNA. Random amplified polymorphic DNA method (RAPD) revealed a difference between C. macranthos var. rebunense from others. That C. calceolus and C. macranthos which were both collected in the Eurasia differed was demonstrated by their rbcL analyses and ITS sequences. The genetic analyses confirmed that C. × ventricosum was a hybrid of C. calceolus × C. macranthos. Thus, analyses using rbcL and ITS region are useful to distinguish species and varieties of Cypripedium.