Nickel elicits a fast antioxidant response in Coffea arabica cells
Nickel elicits a fast antioxidant response in Coffea arabica cells
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DOI：
10.1016/j.plaphy.2006.06.002
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发表时间：
2006-05-01
影响因子：
6.5
通讯作者：
Azevedo, R. A.
中科院分区：
文献类型：
--
作者：
Gomes-Junior, R. A.;Moldes, C. A.;Azevedo, R. A.
咖啡（Coffea arabica L.）研究了细胞悬浮培养物对镍（Ni）的耐受性。Ni在细胞中非常迅速地积累，并且积累可以与培养基中NiCl 2浓度的增加直接相关。在0.05 mM NiCl 2下刺激生长，但在0.5 mM NiCl 2下，生长速率降低。通过在0.5 mM NiCl 2下脂质过氧化反应的增加检测到活性氧存在下的改变的指示。过氧化氢酶（CAT; EC 1.11.1.6）、谷胱甘肽还原酶（GR; EC 1.6.4.2）、抗坏血酸过氧化物酶（APX; EC 1.11.1.11）、愈创木酚过氧化物酶（GOPX; EC 1.11.1.7）和超氧化物歧化酶（SOD; EC 1.15.1.1）的活性增加，尤其是在较早的NiCl 2暴露时间，并且对于大多数测试的暴露时间，0.5 mM NiCl 2的活性更高。非变性聚丙烯酰胺凝胶电泳显示CAT同工酶1种，SOD同工酶9种，GR同工酶4种。NiCl 2处理对SOD同工酶的影响不同，其中一种GR同工酶被NiCl 2增加。NiCl 2在0.05 mM不诱导脂质过氧化反应，主要反应似乎是通过诱导SOD，CAT，GOPX和APX活性的活性氧的去除，并通过诱导GR，以确保还原型谷胱甘肽的可用性。(c)2006年爱思唯尔SAS。All rights reserved.
The antioxidant responses of coffee (Coffea arabica L.) cell suspension cultures to nickel (Ni) were investigated. Ni was very rapidly accumulated in the cells and the accumulation could be directly correlated with the increase of NiCl2 concentration in the medium. At 0.05 MM NiCl2 growth was stimulated, but at 0.5 mM NiCl2, the growth rate was reduced. An indication of alterations in the presence of reactive oxygen species was detected by an increase in lipid peroxidation at 0.5 mM NiCl2. Catalase (CAT; EC 1.11.1.6), glutathione reductase (GR; EC 1.6.4.2), ascorbate peroxidase (APX; EC 1.11.1.11), guaiacol peroxidase (GOPX; EC 1.11.1.7) and superoxide dismutase (SOD; EC 1.15.1.1) activities were increased, particularly at earlier NiCl2 exposure times and the activities were higher at 0.5 mM NiCl2 for most of exposure times tested. Non-denaturing PAGE revealed one CAT isoenzyme, nine SOD isoenzymes and four GR isoenzymes. The SOD isoenzymes were differentially affected by NiCl2 treatment and one GR isoenzyme was increased by NiCl2. NiCl2 at 0.05 mM did not induce lipid peroxidation and the main response appeared to be via the induction of SOD, CAT, GOPX and APX activities for the removal of the reactive oxygen species and through the induction of GR to ensure the availability of reduced glutathione. (c) 2006 Elsevier SAS. All rights reserved.