The effect of growth differentiation factor-5, 6, 7 in chondrogenic cell differentiation of ATDC-5
The effect of growth differentiation factor-5, 6, 7 in chondrogenic cell differentiation of ATDC-5
复制标题
生长分化因子5、6、7对ATDC-5软骨形成细胞分化的影响
DOI：
10.11648/j.ajbio.20140205.13
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Sawa Y.
中科院分区：
文献类型：
--
作者：
Hatakeyama Y;Matsuda Y;Hatakeyama J;Oka K;Anan H;Tsuruga E;Inai T;Ishikawa H;Sawa Y.
同源异型盒基因存在于意大利蜜蜂的基因组中，并且它们的表达主要在胚胎发育期间进行了研究。本研究的目的是评估三个关键hox基因从幼虫期到成虫期的表达。蜜蜂GB 51301，Ant和Ubx的表达在24和72小时的幼虫和蛹中进行了检查，根据眼睛的颜色进行分类，使用定量RT PCR在整个身体或仅头部。结果表明，这些基因的表达水平随着各种器官的发育而变化。GB 51301（HOX 3/zen）表达主要定位于头部，并且在第一神经细胞形成时在幼虫中表达，并且在成虫中表达。ANTP在白眼蛹中的表达时间与触角的发育时间相似。UBX在白色和红眼脓疱中也表达，表达时间与腿形成时间相似。当这些器官发育完成时，这些基因在棕色眼蛹中几乎不表达。这些基因主要存在于人体内。
The homeobox genes are present in the genome of the honeybee Apis mellifera and their expression has been studied principally during embryonic development. The aim of this research was to evaluate expression of three key hox genes from the larval period to the adult. The expression of honeybee GB51301, Ant and Ubx were examined in 24 and 72 hrs larvae, and pupa, classified on the basis of eye colours, using quantitative RT PCR on either the entire body or the head only. The results show that levels of expression from these genes change in relation to the development of various organs. GB51301(HOX3/zen) expression is mainly localised in the head and is expressed in the larvae when the first nervous cells are formed and in the adult worker. ANTP is expressed in the white-eye pupa at a similar time to the development of antenna. UBX is also expressed in white and red eye pupae at a similar time to leg formation. The expression of these genes is practically absent in the brown eye pupa when the development of these organs is completed. These genes are found mainly in the body.