Anatomical identification of neurons in selected brain regions associated with maternal behavior deficits induced by knife cuts of the lateral hypothalamus in rats.
Anatomical identification of neurons in selected brain regions associated with maternal behavior deficits induced by knife cuts of the lateral hypothalamus in rats.
复制标题
解剖学鉴定与大鼠下丘脑外侧刀割引起的母性行为缺陷相关的选定大脑区域中的神经元。
DOI：
10.1002/cne.902370411
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Pfaff,DW
中科院分区：
文献类型：
--
作者：
Numan,M;Morrell,JI;Pfaff,DW
本实验确定了与大鼠下丘脑外侧（LH）损伤引起的母性行为缺陷相关的神经元。完全母性哺乳大鼠在腹内侧核水平的背侧或腹侧 LH 处接受双侧冠状刀切割。用于切割的钢丝刀的刀片涂有辣根过氧化物酶（HRP）。术后 4 天对所有雌性的母性行为进行了研究，然后对大脑进行处理，以定位逆行填充 HRP 的神经元。分析的重点是那些被认为对母亲行为重要的大脑区域。背侧 LH 切割严重扰乱了母亲的行为，而腹侧 LH 切割却没有。与背侧切口相比，腹侧切口用 HRP 标记了更多的内侧视前区 (MPOA) 和间隔对角带神经元。与腹侧切口相比，背侧 LH 切口在外侧视前区 (LPOA)、大细胞视前区、终纹床核、腹侧被盖区 (VTA)、黑质和中央灰质中用 HRP 标记了更多的神经元。先前的研究表明，MPOA 的外侧传出神经与 VTA 相互作用，控制母体行为。本实验的结果表明，通过腹侧 LH 直接下降到脑干的 MPOA 轴突对于母性行为并不是必需的。我们的结果与 MPOA-to-LPOA-to-VTA 回路可能对母亲行为很重要的观点一致。
The present experiment identified neurons associated with maternal behavior deficits induced by damage to the lateral hypothalamus (LH) in rats. Fully maternal lactating rats received bilateral coronal knife cuts through either the dorsal or ventral LH at the level of the ventromedial nucleus. The blade of the wire knife used to make the cuts was coated with horseradish peroxidase (HRP). The maternal behavior of all females was studied for 4 days postoperatively and then the brains were processed for the localization of neurons retrogradely filled with HRP. The analysis focused on those brain regions thought to be important for maternal behavior.The dorsal LH cuts severely disrupted maternal behavior while the ventral LH cuts did not. The ventral cuts labeled more medial preoptic area (MPOA) and septal‐diagonal band neurons with HRP than did the dorsal cuts. The dorsal LH cuts labeled more neurons with HRP in the lateral preoptic area (LPOA), magnocellular preoptic area, bed nucleus of the stria terminalis, ventral tegmental area (VTA), substantia nigra, and central gray than did the ventral cuts.Previous research has suggested that the lateral efferents of the MPOA interact with the VTA in the control of maternal behavior. The results of the present experiment suggest that MPOA axons which descenddirectlyto the brainstem via the ventral LH are not essential for maternal behavior. Our results are consistent with the view that an MPOA‐to‐LPOA‐to‐VTA circuit may be important for maternal behavior.