A model for incorporating novel alleles from the primary gene pool into elite crop breeding programs while reselecting major genes for domestication or adaptation
A model for incorporating novel alleles from the primary gene pool into elite crop breeding programs while reselecting major genes for domestication or adaptation
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DOI：
10.1071/cp08223
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发表时间：
2009-01-01
影响因子：
1.9
通讯作者：
Falk, D. E.
中科院分区：
文献类型：
--
作者：
Cowling, W. A.;Buirchell, B. J.;Falk, D. E.
成本、时间、连锁阻力和遗传漂变不利于将来自外来或非适应种质的潜在有价值的等位基因掺入优良作物植物中，特别是对于数量性状。我们提出了一个模型，窄叶羽扇豆（Lupinus angustifolius）的动机，有效地将新的等位基因从异国情调或非适应的捐助者到精英基因池在两个阶段的育种。在阶段1中，来自二项分布的概率函数提供了至少95%的置信度，即潜在有价值的供体等位基因（A '）将在与优良品系回交的两个循环中存活，并且在BC2衍生的品系中固定。在回交过程中，来自优良亲本的多达6个主要驯化或适应基因被重新选择，并在BC2S0：1家系中纯合。BC2S0：1中的每株植物平均含有12.5%的供体等位基因，其中特定供体等位基因在BC2S0：1群体中的至少一株完全驯化植物中是纯合的概率>95%。选择这些行或随后的田间试验中的植物的有价值的数量性状，并杂交成优良种质以开始阶段2。第一阶段是快速和相对较低的成本，并提供了一个新的遗传多样性进入精英育种池的连续流。
Cost, time, linkage drag, and genetic drift work against the incorporation of potentially valuable alleles from exotic or non-adapted germplasm into elite crop plants, particularly for quantitative traits. We present a model, motivated by narrow-leafed lupin (Lupinus angustifolius), for efficient incorporation of new alleles from exotic or non-adapted donors into elite gene pools during two phases of breeding. In Phase 1, probability functions from the binomial distribution provide at least 95% confidence that a potentially valuable donor allele (A') will survive two cycles of backcrossing to elite lines and is fixed in BC2-derived lines. During backcrossing, up to 6 major domestication or adaptation genes from the elite parents are reselected and made homozygous in BC2S0 : 1 family rows. Each plant in the BC2S0 : 1 contains on average 12.5% donor alleles, with >95% probability that a particular donor allele is homozygous in at least one fully domesticated plant in the BC2S0 : 1 population. Plants in these rows or subsequent field trials are selected for valuable quantitative traits, and crossed into elite germplasm to commence Phase 2. Phase 1 is rapid and relatively low cost, and provides a continuous flow of novel genetic diversity into the elite breeding pool.