Photochemical crosslinking of soluble wool keratins produces a mechanically stable biomaterial that supports cell adhesion and proliferation
Photochemical crosslinking of soluble wool keratins produces a mechanically stable biomaterial that supports cell adhesion and proliferation
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DOI：
10.1002/jbm.a.32913
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发表时间：
2010-12-01
影响因子：
4.9
通讯作者：
Elvin, Christopher M.
中科院分区：
文献类型：
--
作者：
Sando, Lillian;Kim, Misook;Elvin, Christopher M.
从各种“硬组织”如羊毛、头发和指甲中提取的角蛋白作为丰富的生物相容性材料的来源越来越多地被研究。在这项研究中，我们探讨了最近的光化学方法交联溶解羊毛角化病，目的是生产一个机械上有利的生物材料。通过氧化二硫化物并提取所得可溶性角化物来分离羊毛蛋白。通过液相色谱-质谱法分析α-和γ-角蛋白组分以鉴定其组成蛋白。通过蓝光、钌络合物和过硫酸盐催化的光氧化过程，通过α-角化病的共价交联制备水凝胶。通过高效液相色谱和质谱分析证明了双酪氨酸交联的存在。交联α-角蛋白材料具有适度的拉伸强度和弹性，以及较高的粘附强度。该材料在交联后显示出适度的收缩，然而可以通过在2.5%甘油存在下交联来防止收缩，从而产生不收缩或溶胀的凝胶。小溶质如三羟甲基氨基甲烷和甘油影响交联材料的交联密度和弹性模量。α-角蛋白能够支持NIH/3 T3成纤维细胞在体外的粘附和生长。通过这种简单的光交联方法制备机械稳定的角蛋白生物材料可能适用于各种组织工程应用。(C)2010 Wiley Periodicals，Inc. J Biomed Mater Res Part A：95A：901-911，2010.
Keratins extracted from various "hard tissues" such as wool, hair, and nails are increasingly being investigated as a source of abundant, biocompatible materials. In this study we explored a recent photochemical method to crosslink solubilized wool keratoses, with the aim of producing a mechanically favorable biomaterial. Wool proteins were isolated by oxidizing the disulfides and extracting the resulting soluble keratoses. The alpha- and gamma-keratose fractions were analyzed by liquid chromatography-mass spectrometry to identify their constituent proteins. Hydrogels were produced by covalent crosslinking of the alpha-keratoses via a photo-oxidative process catalyzed by blue light, a ruthenium complex, and persulfate. The presence of dityrosine crosslinks was demonstrated by high performance liquid chromatography and mass spectrometry analyses. The crosslinked alpha-keratose material had moderate tensile strength and elasticity, and high adhesive strength. The material displayed modest shrinking after crosslinking, however the shrinking could be prevented by crosslinking in the presence of 2.5% glycerol, resulting in gels that did not shrink or swell. Small solutes such as Tris and glycerol influenced the crosslink density and elastic modulus of the crosslinked material. The alpha-keratose was able to support adhesion and growth of NIH/3T3 fibroblasts in vitro. The fabrication of mechanically stable keratin biomaterials by this facile photo-crosslinking method may be useful for various tissue engineering applications. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 901-911, 2010.