Development and characterization of salivary adenoid cystic carcinoma cell line.
Development and characterization of salivary adenoid cystic carcinoma cell line.
复制标题
DOI：
10.1016/j.oraloncology.2014.06.012
复制
发表时间：
2014-10
期刊：
影响因子：
4.8
通讯作者：
El-Naggar AK
中科院分区：
文献类型：
--
作者：
Li J;Perlaky L;Rao P;Weber RS;El-Naggar AK
建立体外培养的腺样囊性癌细胞系，作为功能研究的替代物。体外培养舌根部原代ACC细胞，h-TERT永生化。进行形态、细胞遗传学和功能研究。通过角蛋白和平滑肌肌动蛋白的阳性反应以及细胞和核的表型特征来证实肿瘤细胞。体外细胞生长和集落形成实验支持其肿瘤性质。我们鉴定了一种具有上皮-肌上皮混合功能的ACC细胞系，作为功能实验的资源。
To develop in-vitro adenoid cystic carcinoma cell line as a surrogate for functional studies. Cells obtained from a primary ACC of the base of tongue were cultivated in-vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed. Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature. We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.
影响因子：
4
作者：
Leung, Cheuk T.
通讯作者：
Leung, Cheuk T.