Honey bee (Apis mellifera) transferrin-gene structure and the role of ecdysteroids in the developmental regulation of its expression
Honey bee (Apis mellifera) transferrin-gene structure and the role of ecdysteroids in the developmental regulation of its expression
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DOI：
10.1016/j.ibmb.2003.12.003
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发表时间：
2004-05-01
影响因子：
3.8
通讯作者：
Hartfelder, K
中科院分区：
文献类型：
--
作者：
do Nascimento, AM;Cuvillier-Hot, V;Hartfelder, K
社会生活容易受到微生物的入侵，转铁蛋白与铁离子的结合是限制微生物获得铁的有效策略。在这项研究中，我们分离的cDNA和基因组克隆编码意大利蜜蜂转铁蛋白（AmTRF）基因。它有一个2136 bp的开放阅读框（ORF），分布在9个外显子上。推导的蛋白质序列包括686个氨基酸残基加上26个残基的信号序列，给出了76 kDa的预测分子量。与已知的昆虫转铁蛋白的推导的AmTRF氨基酸序列的比较显示显着的相似性延伸到整个序列。它与单铁转铁蛋白簇合，在N-末端叶中与单铁转铁蛋白共享推定的铁结合残基。在蜜蜂发育中AmTRF表达的功能分析中，我们监测了其在幼虫和蛹阶段的表达谱。AmTRF的负调控蜕皮类固醇推导出的发育表达谱证实了实验治疗的纺纱阶段蜜蜂幼虫与20-羟基蜕皮激素，和保幼激素的第四龄幼虫。相反，将保幼激素应用于旋转阶段的幼虫，对AmTRF转录水平只有很小的影响。这是第一个研究涉及蜕皮甾体在转铁蛋白表达的发育调节昆虫物种。(C)2003 Elsevier Ltd.保留所有权利。
Social life is prone to invasion by microorganisms, and binding of ferric ions by transferrin is an efficient strategy to restrict their access to iron. In this study, we isolated cDNA and genomic clones encoding an Apis mellifera transferrin (AmTRF) gene. It has an open reading frame (ORF) of 2136 bp spread over nine exons. The deduced protein sequence comprises 686 amino acid residues plus a 26 residues signal sequence, giving a predicted molecular mass of 76 kDa. Comparison of the deduced AmTRF amino acid sequence with known insect transferrins revealed significant similarity extending over the entire sequence. It clusters with monoferric transferrins, with which it shares putative iron-binding residues in the N-terminal lobe. In a functional analysis of AmTRF expression in honey bee development, we monitored its expression profile in the larval and pupal stages. The negative regulation of AmTRF by ecdysteroids deduced from the developmental expression profile was confirmed by experimental treatment of spinning-stage honey bee larvae with 20-hydroxyecdysone, and of fourth instar-larvae with juvenile hormone. A juvenile hormone application to spinning-stage larvae, in contrast, bad only a minor effect on AmTRF transcript levels. This is the first study implicating ecdysteroids in the developmental regulation of transferrin expression in an insect species. (C) 2003 Elsevier Ltd. All rights reserved.