PpAKR1A, a Novel Aldo-Keto Reductase from Physcomitrella Patens, Plays a Positive Role in Salt Stress
PpAKR1A, a Novel Aldo-Keto Reductase from Physcomitrella Patens, Plays a Positive Role in Salt Stress
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PpAKR1A 是一种来自小立碗藓的新型醛酮还原酶，在盐胁迫中发挥积极作用
DOI：
10.3390/ijms20225723
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发表时间：
2019-11-01
影响因子：
5.6
通讯作者：
He, Yikun
中科院分区：
文献类型：
--
作者：
Chen, Lu;Bao, Fang;He, Yikun
苔藓小立碗藓（Physcomitrella patens）耐受高盐环境。在植物中，盐胁迫可能会诱导有毒反应性羰基物质（RCS）的产生和氧化损伤。醛酮还原酶（AKR）是参与RCS解毒的一大类NADP依赖性氧化还原酶。然而，这个超家族中的许多成员仍然没有特征。在这项研究中，我们克隆并表征了一个假定的AKR1从P.patens，命名为PpAKR1A。值得注意的是，PpAKR1A的转录水平诱导盐和甲基乙二醛（MG）胁迫，和重组PpAKR1A蛋白催化还原有毒醛。PpAKR1A基因敲除的展叶商陆突变体（ppakr1a）对NaCl和MG处理敏感，表现为叶绿素浓度比野生型低得多，MG和H2O2浓度比野生型高得多。同时，ppakr1a植株在盐胁迫下表现出降低的MG还原活性和活性氧清除能力，这可能是由于超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）等抗氧化酶活性降低所致。我们的研究结果表明，PpAKR1A是一种醛酮还原酶，可以解毒MG，因此在P.patens的盐胁迫耐受性中发挥重要作用。
The moss Physcomitrella patens is tolerant of highly saline environments. In plants, salinity stress may induce the production of toxic reactive carbonyl species (RCS) and oxidative damage. Aldo-keto reductases (AKRs) are a large group of NADP-dependent oxidoreductases involved in RCS detoxification. However, many members in this superfamily remain uncharacterized. In this study, we cloned and characterised a putative AKR1 from P. patens, named PpAKR1A. Notably, the transcription level of PpAKR1A was induced by salt and methylglyoxal (MG) stress, and the recombinant PpAKR1A protein catalysed the reduction of toxic aldehydes. PpAKR1A knockout mutants of P. patens (ppakr1a) were sensitive to NaCl and MG treatment, as indicated by much lower concentrations of chlorophyll and much higher concentrations of MG and H2O2 than those in WT plants. Meanwhile, ppakr1a plants exhibited decreases in the MG-reducing activity and reactive oxygen species-scavenging ability in response to salt stress, possibly due to decreases in the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). Our results indicate that PpAKR1A is an aldo-keto reductase that detoxifies MG and thus plays an important role in salt stress tolerance in P. patens.