A fork head/HNF-3 homolog expressed in the pharynx and intestine of the Caenorhabditis elegans embryo
A fork head/HNF-3 homolog expressed in the pharynx and intestine of the Caenorhabditis elegans embryo
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DOI：
10.1006/dbio.1996.0219
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发表时间：
1996-09-15
影响因子：
2.7
通讯作者：
McGhee, JD
中科院分区：
文献类型：
--
作者：
Azzaria, M;Goszczynski, B;McGhee, JD
我们已经克隆了一个成员的叉头/HNF-3家族的转录因子从线虫秀丽隐杆线虫。在预测的DNA结合结构域中，这种基因称为Ce-Fkh-1，与果蝇叉头和大鼠肝脏HNF-3 α，β和γ基因具有75-78%的相同性。Ce-Fkh-1 mRNA在胚胎中高度富集。Ce-Fh-1基因产生三种主要的转录物：最长的mRNA保留其原始的5 '端，但两种较短的mRNA分别在外显子2和3的开始处反式剪接。原位杂交和转基因Ce-Fkh-1：：lacZ报告基因构建表明Ce-Fh-1基因在胚胎的咽和肠中表达，开始于增殖中期阶段。Ce-Fh-1表达的第二阶段发生在幼虫体细胞性腺的细胞中。Ce-Fkh-1在C.将线虫胚胎中叉头的表达与果蝇胚胎整个肠道中叉头的表达以及哺乳动物胚胎内胚层中HNF-3（α β γ）的表达进行比较。这种保守的基因表达模式指向原肠胚形成和消化道形成的普遍特征。(C)出版社：Academic Press，Inc.
We have cloned a member of the fork head/HNF-3 family of transcription factors from the nematode Caenorhabditis elegans. Within the predicted DNA binding domain, this gene, called Ce-Fkh-1, is 75-78% identical to the Drosophila fork head and rat liver HNF-3 alpha, beta, and gamma genes. Ce-Fkh-1 mRNA is highly enriched in embryos. The Ce-Fh-1 gene produces three major transcripts: the longest mRNA retains its original 5'-end but two shorter mRNAs are trans-spliced at the beginning of exons 2 and 3, respectively. In situ hybridization and transgenic Ce-Fkh-1::lacZ reporter constructs indicate that the Ce-Fh-1 gene is expressed in both pharynx and intestine of the embryo, beginning at the midproliferation stage. A second phase of Ce-Fh-1 expression occurs in cells of the larval somatic gonad. The pharynx-gut expression of Ce-Fkh-1 in the C. elegans embryo is compared with expression of fork head throughout the gut of Drosophila embryos and with expression of HNF-3 (alpha beta gamma) in the endoderm of mammalian embryos. Such conserved patterns of gene expression point to universal features of gastrulation and of digestive tract formation. (C) 1996 Academic Press, Inc.