Biological Effects of Hyaluronic Acid-Based Dermal Fillers and Laser Therapy on Human Skin Models
Biological Effects of Hyaluronic Acid-Based Dermal Fillers and Laser Therapy on Human Skin Models
复制标题
DOI：
10.36849/jdd.2020.4856
复制
发表时间：
2020-09-01
影响因子：
1.5
通讯作者：
Huth, Sebastian
中科院分区：
文献类型：
--
作者：
Huth, Laura;Marquardt, Yvonne;Huth, Sebastian
皮肤填充物的注射是最常进行的美容程序之一，本研究的目的是调查不同的稳定透明质酸（HA）和聚-L-乳酸填充物的生物学效应，并没有随后的额外的分数激光共治疗皮肤形态和基因表达。皮内注射导致组织学分析检测到的表皮厚度显著增加。结合HA填料与消融分数CO2-或Er：YAG激光照射增强了这种效果。基因表达谱显示组织重塑（例如TIMP 3、SERPIN E1）和胶原蛋白（COL 11 A1）的调节剂的上调。另一方面，我们检测到分化标志物（例如FLG、LOR、KRT 1）和促炎细胞因子IL-36、IL-113）的下调。有趣的是，基于HA的填充物揭示了趋化因子如CXCL 5和CCL 20的特异性上调模式，这表明这些填充物对皮肤免疫细胞，特别是单核细胞和巨噬细胞的次级作用。此外，我们的研究结果揭示了分步消融激光治疗和HA真皮填充物注射的协同效应，这表明两种治疗的组合。
Injection of dermal fillers is one of the most frequently performed aesthetic procedures.The aim of the present study was to investigate the biological effects of different stabilized hyaluronan (HA) and poly-l -lactic acid fillers with and without subsequent additional fractional laser co-treatment on skin morphology and gene expression. Intradermal injection resulted in a significant enhancement of epidermal thickness detected by histological analysis. Combining HA fillers with ablative fractional CO2- or Er:YAG laser irradiation enhanced this effect. Gene expression profiling revealed an upregulation of modulators of tissue remodeling (eg TIMP3, SERPIN El) and collagens (COL11A1). On the other hand, we detected a downregulation of differentiation markers (eg FLG, LOR, KRT1) and proinflammatory cytokines IL-36, IL-113). Interestingly, HA-based fillers revealed a specific upregulation pattern of chemokines such as CXCL5 and CCL20 suggesting a secondary effect of these fillers on the immune cells of the skin, especially monocytes and macrophages.Taken together, our data show enhancing effects of dermal fillers on epidermal thickness and prove the proliferating effects of these products on epidermal cells on the molecular level. Moreover, our findings reveal synergistic effects of fractional ablative laser treatment and HA dermal filler injection suggesting a combination of both treatments.