Localization of Merkel cells at hairless and hairy human skin sites using keratin 18
Localization of Merkel cells at hairless and hairy human skin sites using keratin 18
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DOI：
10.1139/o95-070
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发表时间：
1995-09-01
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影响因子：
--
通讯作者：
Germain, L
中科院分区：
文献类型：
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作者：
Fradette, J;Godbout, MJ;Germain, L
默克尔细胞是皮肤的神经分泌细胞，具有上皮特征，如桥粒和角蛋白8、18、19和20的表达。梅克尔细胞在成人皮肤中几乎没有分布。虽然它们存在于毛囊中，但在无毛的解剖部位，如手掌和脚底，它们的密度更高。这些细胞通常受感觉神经纤维支配，被认为是特化的机械感觉皮肤受体细胞。然而，它们的确切起源和功能尚不清楚。本研究的目的是用免疫组织化学方法分别靶向角蛋白18和19的抗体Ks18.174和Ks19.1定位人无毛和有毛皮肤中的默克尔细胞。在手掌和脚底的无毛皮肤中，默克尔细胞已定位于表皮基底层的网脊底部。为了研究毛发解剖部位的默克尔细胞分布，我们选择了乳房皮肤，这是一种包含小毛囊的组织，是覆盖身体大部分表面的典型组织。滤泡间表皮存在默克尔细胞。在毛囊中，已经在峡部区域发现了它们。
Merkel cells are neurosecretory cells of the skin with epithelial features such as desmosomes and expression of keratins 8, 18, 19, and 20. Merkel cells are scarcely distributed in adult human skin. Although they are present in hair follicles, their density is higher at hairless anatomic sites such as palms and soles. These cells are often innervated by sensory nerve fibers and are thought to be specialized mechanosensory skin receptor cells. However, their precise origin and function are not clearly established. The aim of this study was to localize Merkel cells in human hairless and hairy skin by immunohistochemistry with antibodies Ks18.174 and Ks19.1 directed against keratins 18 and 19, respectively. In glabrous skin of palm and sole, Merkel cells have been localized at the bottom of the rete ridges, in the epidermal basal layer. To study Merkel cell distribution at hairy anatomic sites, we have chosen breast skin, a tissue containing small hair follicles typical of those covering most of the body's surface. Merkel cells were present in the interfollicular epidermis. In hair follicles, they have been identified in the isthmus region.