Induction of high titers of anti-IgE by immunization of inbred mice with syngeneic IgE.
Induction of high titers of anti-IgE by immunization of inbred mice with syngeneic IgE.
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通过用同基因 IgE 免疫近交小鼠诱导高滴度抗 IgE。
DOI：
10.1073/pnas.84.14.5009
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发表时间：
1987
影响因子：
11.1
通讯作者：
Nisonoff,A
中科院分区：
文献类型：
--
作者：
Haba,S;Nisonoff,A
我们通过用与匙孔血蓝蛋白缀合的同源单克隆 IgE 免疫 A/J 小鼠，产生了针对 IgE 同型决定簇的高滴度（高达 1 mg/ml）抗体。同时诱导每毫升多达3毫克的抗独特型抗体。与传统的类风湿因子相比，抗同种型抗体具有中等高的亲和力 (10(7)-10(8) M-1)。抗 IgE 抗血清测定表明存在 IgE，无论是游离的还是免疫复合物的形式；由于同型决定因素被掩盖，后一个值是最低估计值。现在可以研究这些高滴度抗 IgE 的调节作用。单克隆、同基因抗 IgE 抗体的可用性将促进此类研究。
We have generated high titers (up to 1 mg/ml) of antibodies to isotypic determinants of IgE by immunization of A/J mice with syngeneic monoclonal IgE conjugated to keyhole limpet hemocyanin. As much as 3 mg of anti-idiotypic antibodies per ml was induced at the same time. In contrast to conventional rheumatoid factors, the anti-isotypic antibodies are of moderately high affinity (10(7)-10(8) M-1). Assays of the anti-IgE antisera indicated the presence of IgE, both free and in the form of immune complexes; the latter values are minimum estimates owing to masking of isotypic determinants. Regulatory effects of these high titers of anti-IgE can now be investigated. Such studies will be facilitated by the availability of monoclonal, syngeneic anti-IgE antibodies.