High segregation distortion in maize B73 x teosinte crosses.
High segregation distortion in maize B73 x teosinte crosses.
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DOI：
10.4238/2012.march.19.3
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
G. Wang;Q. He;Z. Xu;R. Song
中科院分区：
文献类型：
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作者：
G. Wang;Q. He;Z. Xu;R. Song
构建了栽培玉米自交系和特辛特种的遗传连锁图谱。其中一个群体由玉米自交系B73与小粒玉米杂交产生的81个F(2)单株组成，另一个群体由63个回交单株组成，回交单株来自玉米自交系B73与二倍体玉米的杂交。在B73×Zays SSP parviglumis F(2)群体中，172个简单序列重复标记被定位在10条染色体上，总长度为2210.8 cM。在B73×玉米双交回交群体中，258个SSR标记被定位在10条染色体上，总长度为1357.7 cM。两个图谱的比较表明，二粒小麦的图谱总长度为1357.7 cM，约为小叶大麦的61.4%(2210.8 cM)。在B73×小黑麦F(2)群体的第1、2、3、5、6、7和10号染色体上，以及在B73×小黑麦回交群体的1-5和8-10染色体上，发现了广泛的分离扭曲区域。分离扭曲分析表明，种间群体B73×Zays SSP的分离扭曲比高于B73×Zays SSP的分离偏差。我们发现，在玉米栽培种和野生种之间的遗传杂交中，重组距离是高度可变的。
Two genetic linkage maps of cultivated maize inbred lines and teosinte species were constructed. One population comprised 81 F(2) individuals derived from a cross between maize inbred line B73 and Zea mays ssp parviglumis, while the second consisted of 63 backcross individuals from a cross of maize inbred line B73 with Z. mays ssp diploperennis. In the B73 x Z. mays ssp parviglumis F(2) population, 172 simple sequence repeat (SSR) markers were mapped to 10 chromosomes, which covered 2210.8 cM. In the B73 x Z. mays ssp diploperennis backcross population, 258 SSR markers were mapped to 10 chromosomes, covering 1357.7 cM. Comparison of the two maps revealed that the total map length of Z. mays ssp diploperennis covers 1357.7 cM, which is about 61.4% of that of Z. mays ssp parviglumis (2210.8 cM). Extensive segregation distortion regions were found on chromosomes 1, 2, 3, 5, 6, 7, and 10 in the B73 x Z. mays ssp parviglumis F(2) population and on chromosomes 1-5 and 8-10 in the B73 x Z. mays ssp parviglumis backcross population. Segregation distortion analysis confirmed that the segregation distortion ratio in the interspecific population B73 x Z. mays ssp diploperennis was higher than in B73 x Z. mays ssp parviglumis. We found that the recombination distances are highly variable in these genetic crosses between cultivated and wild species of maize.