Function of subunits within the eighth component of human complement: selective removal of the gamma chain reveals it has no direct role in cytolysis.
Function of subunits within the eighth component of human complement: selective removal of the gamma chain reveals it has no direct role in cytolysis.
复制标题
人类补体第八个组成部分中亚基的功能：选择性去除伽玛链表明它在细胞溶解中没有直接作用。
DOI：
10.1021/bi00300a008
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Sodetz,JM
中科院分区：
文献类型：
--
作者：
Brickner,A;Sodetz,JM
放大图片作者：James M. Sodetz* 摘要：人补体的第八种成分（C8）由二硫键连接的Ay二聚体组成，Ay二聚体与补体非共价结合。本实验室先前的结果表明，其中两个亚基在C8的细胞溶解功能中具有不同的作用。C8与膨胀性C5 b-7复合物的结合严格通过α介导，而C8与靶膜的脂质双层之间的相互作用主要通过α介导。在本研究中，我们通过表征C8 '（一种缺乏y的C8衍生物）的功能特性来研究y亚基在细胞溶解中的重要性。该衍生物的制备通过在纯化的ay中有限地裂解二硫键并将a与7分离来完成。当混合时，纯化的α和β结合形成C8 ′。当测试与正常C8的功能相似性时，获得以下结果。(1)特异性和可饱和
Avram Brickner and James M. Sodetz* abstract: The eighth component of human complement (C8) consists of a disulfide-linked ay dimer that is noncovalently associated with ß. Previous results from this laboratory es-tablished that two of these subunits have distinct roles in the cytolytic function of C8. Binding of C8 to the precursive C5b-7 complex is mediated strictly through ß while interaction between C8 and the lipid bilayer of target membranes occurs primarily through a. In the present study, we examined the importance of the y subunit in cytolysis by characterizing functional properties of C8', a derivative of C8 that lacks y. Preparation of this derivative was accomplished by limited cleavage of disulfide bonds in purified ay and separation of a from 7. When mixed, purified a and ß combined to form C8'. When tested for functional similarity to normal C8, the following results were obtained.(1) Specific and saturable