Inflammation and angiogenesis in osteoarthritis
Inflammation and angiogenesis in osteoarthritis
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DOI：
10.1002/art.11094
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发表时间：
2003-08-01
影响因子：
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通讯作者：
Walsh, DA
中科院分区：
文献类型：
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作者：
Haywood, L;McWilliams, DF;Walsh, DA
Objective.目的：探讨骨关节炎（OA）患者滑膜组织中炎症反应与血管生成的关系。对104例符合美国流变学学会OA标准并接受全关节置换术或关节镜检查的患者进行苏木精和伊红染色和炎症组织学分级。从70例患者中获得的滑膜标本的目的性样本用于进一步分析。免疫组化检测血管内皮细胞、内皮细胞增殖核、巨噬细胞和血管内皮生长因子。血管生成（EC增殖，EC分数面积），巨噬细胞分数面积和VEGF免疫反应性进行了测量，使用计算机辅助图像分析。采用免疫荧光组织化学双标法检测VEGF的细胞定位。同时评估膝关节间隙狭窄和骨赘形成的放射学评分。104例OA患者中32例（31%）的滑膜组织样本显示重度炎症;常观察到内膜增厚和相关淋巴聚集体。EC面积分数、EC增殖和VEGF免疫反应性均随组织学炎症分级和巨噬细胞面积分数的增加而增加。在滑膜内膜衬里，VEGF免疫反应定位于巨噬细胞，并增加EC分数面积和血管生成。无炎症或血管生成指数与放射学评分显著相关。滑膜中的炎症和血管生成与OA相关。由发炎滑膜产生的血管生成生长因子VEGF可促进血管生成，从而促进OA中的炎症。
Objective. To quantify the relationship between inflammation and angiogenesis in synovial tissue from patients with osteoarthritis (OA).Methods. Hematoxylin and eosin staining and histologic grading for inflammation were performed for 104 patients who met the American College of Rheumatology criteria for OA and had undergone total joint replacement or arthroscopy. A purposive sample of synovial specimens obtained from 70 patients was used for further analysis. Vascular endothelium, endothelial cell (EC) proliferating nuclei, macrophages, and vascular endothelial growth factor (VEGF) were detected by immunohistochemical analysis. Angiogenesis (EC proliferation, EC fractional area), macrophage fractional area, and VEGF immunoreactivity were measured using computer-assisted image analysis. Double immunofluorescence histochemical analysis was used to determine the cellular localization of VEGF. Radiographic scores for joint space narrowing and osteophyte formation in the knee were also assessed.Results. Synovial tissue samples from 32 (31%) of 104 patients with OA showed severe inflammation; thickened intimal lining and associated lymphoid aggregates were often observed. The EC fractional area, EC proliferation, and VEGF immunoreactivity all increased with increasing histologic inflammation grade and increasing macrophage fractional area. In the synovial intimal lining, VEGF immunoreactivity was localized to macrophages and increased with increasing EC fractional area and angiogenesis. No inflammation or angiogenic indices were significantly correlated with radiographic scores.Conclusion. Inflammation and angiogenesis in the synovium are associated with OA. The angiogenic growth factor VEGF generated by the inflamed synovium may promote angiogenesis, thereby contributing to inflammation in OA.