Two distinct nuclear stress bodies containing different sets of RNA-binding proteins are formed with HSATIII architectural noncoding RNAs upon thermal stress exposure
Two distinct nuclear stress bodies containing different sets of RNA-binding proteins are formed with HSATIII architectural noncoding RNAs upon thermal stress exposure
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DOI：
10.1016/j.bbrc.2019.06.061
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发表时间：
2019-08-20
影响因子：
3.1
通讯作者：
Hirose, Tetsuro
中科院分区：
文献类型：
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作者：
Aly, Mahmoud Khamis;Ninomiya, Kensuke;Hirose, Tetsuro
核应激体（nSB）是在高度重复的卫星III结构非编码RNA（HSATIII arcRNA）上形成的热应激诱导的无膜核体。在热应激暴露后，HSATIII表达被诱导以螯合特定的RNA结合蛋白组并形成nSB。nSB的主要群体含有SAFB作为标记物，而次要群体为SAFB阴性。在这里，我们发现，HNRNPM，这是以前报道的本地化在核灶附近的SAFB阳性病灶热应力后，本地化在一个小的人口HSATIII依赖的nSB。因此，我们分别使用术语nSB-S和nSB-M来区分SAFB病灶和HNRNPM病灶。分析的nSB的组件显示，每一组包含不同的RNA结合蛋白，包括SLTM和NCO 5A在nSB-Ss和HNRNPA 1和HNRNPH 1在nSB-MS。总体而言，我们的研究结果表明，两组含有HSATIII arcRNA和不同的RNA结合蛋白的nSB热应力暴露后形成。(C)2019爱思唯尔公司All rights reserved.
Nuclear stress bodies (nSBs) are thermal stress-inducible membrane-less nuclear bodies that are formed on highly repetitive satellite Ill architectural noncoding RNAs (HSATIII arcRNAs). Upon thermal stress exposure, HSATIII expression is induced to sequestrate specific sets of RNA-binding proteins and form nSBs. The major population of nSBs contain SAFB as a marker, whereas the minor population are SAFB-negative. Here, we found that HNRNPM, which was previously reported to localize in nuclear foci adjacent to SAFB-positive foci upon thermal stress, localizes in a minor population of HSATIII-dependent nSBs. Hence, we used the terms nSB-S and nSB-M to distinguish the SAFB foci and HNRNPM foci, respectively. Analysis of the components of the nSBs revealed that each set contains distinct RNA-binding proteins, including SLTM and NCO5A in nSB-Ss and HNRNPA1 and HNRNPH1 in nSB-Ms. Overall, our findings indicate that two sets of nSBs containing HSATIII arcRNAs and distinct sets of RNA-binding proteins are formed upon thermal stress exposure. (C) 2019 Elsevier Inc. All rights reserved.