Transient receptor potential ankyrin 1 channels are involved in spontaneous peptide hormone release from astrocytes
Transient receptor potential ankyrin 1 channels are involved in spontaneous peptide hormone release from astrocytes
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瞬时受体电位锚蛋白 1 通道参与星形胶质细胞自发释放肽激素
DOI：
10.1016/j.bbrc.2018.05.097
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发表时间：
2018
影响因子：
3.1
通讯作者：
Tsuboi Takashi
中科院分区：
文献类型：
--
作者：
Takizawa Mai;Harada Kazuki;Nakamura Kazuaki;Tsuboi Takashi
星形胶质细胞是一大群胶质细胞，它们检测神经递质，并通过增加细胞内Ca2+浓度（[Ca2+]i）和释放称为胶质递质的化学分子来做出反应。最近发现的Ca2+内流通过瞬时受体电位锚蛋白1 （TRPA1）通道引起星形胶质细胞自发的[Ca2+]增加。虽然trpa1介导的自发Ca2+信号的一些生理功能已经被揭示，但与胶质递质释放，特别是肽激素分泌的关系在很大程度上是未知的。因此，我们探讨了大鼠星形胶质细胞系C6细胞和原代星形胶质细胞的[Ca2+]陆地胞吐动力学。在C6细胞和原代星形胶质细胞中均观察到trpa1介导的自发[Ca2+]瞬变。全内反射荧光显微镜显示，金星标记的脑源性神经营养因子和神经肽Y从星形胶质细胞中自发释放。TRPA1通道的激活增加了肽激素胞吐的频率，而TRPA1通道的抑制则减少了肽激素胞吐的数量。这些结果表明trpa1介导的自发[Ca2+]增加调节星形胶质细胞肽激素的自发释放。
Astrocytes, a large population of glial cells, detect neurotransmitters and respond by increasing intracellular Ca2+concentration ([Ca2+]i) and releasing chemical molecules called gliotransmitters. Recently discovered Ca2+influx through transient receptor potential ankyrin 1 (TRPA1) channels is reported to cause spontaneous [Ca2+]iincrease in astrocytes. While several physiological functions of TRPA1-mediated spontaneous Ca2+signal have been revealed, relation with gliotransmitter release, especially peptide hormone exocytosis is largely unknown. We therefore explored the [Ca2+]iand exocytosis dynamics in rat astrocyte cell line C6 cells and primary astrocytes. TRPA1-mediated spontaneous [Ca2+]itransients were observed in both C6 cells and primary astrocytes. Total internal reflection fluorescence microscopy revealed that Venus-tagged brain-derived neurotrophic factor and neuropeptide Y were released spontaneously from astrocytes. Activation of TRPA1 channels enhanced the frequency of peptide hormone exocytosis, and inhibition of TRPA1 channels decreased the number of peptide hormone exocytosis. These results suggest that TRPA1-mediated spontaneous [Ca2+]iincrease modulates the spontaneous release of peptide hormones from astrocytes.